发明申请
- 专利标题： Polypeptide
- 专利标题（中）： 多肽
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申请号： US11483220申请日： 2006-07-07
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公开(公告)号： US20070141693A1公开(公告)日： 2007-06-21
- 发明人: Casper Berg , Patrick Maria Derkx , Carol Fioresi , Gijsbert Gerritse , Anja Kellet-Smith , Karsten Kragh , Wei Liu , Andrew Shaw , Bo Sorensen , Charlotte Thoudahl
- 申请人： Casper Berg , Patrick Maria Derkx , Carol Fioresi , Gijsbert Gerritse , Anja Kellet-Smith , Karsten Kragh , Wei Liu , Andrew Shaw , Bo Sorensen , Charlotte Thoudahl
- 主分类号： C12N9/32
- IPC分类号： C12N9/32 ; C12N1/21
摘要：
The invention provides for a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity which may be selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.
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