Expression system for altered expression levels
    3.
    发明授权
    Expression system for altered expression levels 有权
    表达系统改变表达水平

    公开(公告)号:US06225106B1

    公开(公告)日:2001-05-01

    申请号:US09479409

    申请日:2000-01-06

    IPC分类号: C12N1578

    CPC分类号: C07K14/21 C12N9/20 C12N15/78

    摘要: A new expression system is provided which comprises component(s) of a lipase regulation cascade. The lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.

    摘要翻译: 提供了一种新的表达系统,其包含脂肪酶调节级联的组分。 如本文所公开的脂肪酶调节级联包括激酶,DNA结合调节剂,聚合酶,启动子,上游激活序列和分泌因子。 还提供质粒和转化细胞以及使用质粒转化宿主细胞的方法。 此外,提供了可以调节蛋白质的表达的激酶,可调节蛋白质表达的DNA结合调节剂,产碱假单胞菌聚合酶,产碱假马杆菌54启动子,产碱假单胞菌(Pseudomonas alcaligenes)上游激活序列,假单胞菌 产碱分泌因子XcpP,XcpQ,XcpR,XcpS,XcpT,XcpU,XcpV,XcpW,XcpX,XcpY,XcpZ和xcp调节剂OrfV OrfX。

    Lipases with improved surfactant resistance
    6.
    发明授权
    Lipases with improved surfactant resistance 失效
    具有改善的表面活性剂抵抗力的脂肪酶

    公开(公告)号:US6017866A

    公开(公告)日:2000-01-25

    申请号:US732412

    申请日:1996-10-22

    摘要: The present invention provides mutant lipases which retain lipase activity, but have improved surfactant resistance and are thus highly advantageous for use in detergent compositions. Preferred lipases of this type include modified functional forms of a lipase of Pseudomonas alcaligenes having one or more amino acid substitutions at or near the surface of the lipase which effect interaction of the hydrophobic portions of surfactant molecules with initiation sites on the enzyme for surfactant denaturation.

    摘要翻译: PCT No.PCT / EP95 / 01687 Sec。 371日期1996年10月22日第 102(e)日期1996年10月22日PCT提交1995年4月28日PCT公布。 WO95 / 30744PC PCT出版物 日期1995年11月16日本发明提供了保留脂肪酶活性但具有改善的表面活性剂抗性的突变脂肪酶,因此在洗涤剂组合物中的使用非常有利。 这种类型的优选脂肪酶包括在脂肪酶表面处或附近具有一个或多个氨基酸取代的产碱假单胞菌的脂肪酶的修饰的功能形式,其影响表面活性剂分子的疏水部分与用于表面活性剂变性的酶的起始位点的相互作用。

    Polypeptide
    9.
    发明申请
    Polypeptide 审中-公开
    多肽

    公开(公告)号:US20070141693A1

    公开(公告)日:2007-06-21

    申请号:US11483220

    申请日:2006-07-07

    IPC分类号: C12N9/32 C12N1/21

    摘要: The invention provides for a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity which may be selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.

    摘要翻译: 本发明提供可衍生自具有淀粉酶活性的亲本多肽的PS4变体多肽,其可以选自: 146,157,161,178,179,223,229,272,303,307,309和334; (b)包含位置33,34,121,134,141,145,146,157,178,179,223,229,272,303,307和334的氨基酸突变的多肽; (c)包含位置33,34,121,134,141,146,157,178,179,223,229,272,303,307,309和334的氨基酸突变的多肽; 和(d)包含位置3,33,34,70,121,134,141,146,157,178,179,223,229,272,303,307,309和334中的每一个上的氨基酸突变的多肽 ; 参考如SEQ ID NO:1所示的假单胞菌属酵母内切酶序列的位置编号,这种多肽作为食品或饲料添加剂的使用,以及编码其的核酸。