发明申请
US20070143880A1 Methods for Introducing Into a Plant a Polynucleotide of Interest
审中-公开
向植物引入感兴趣的多核苷酸的方法
- 专利标题: Methods for Introducing Into a Plant a Polynucleotide of Interest
- 专利标题(中): 向植物引入感兴趣的多核苷酸的方法
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申请号: US11427371申请日: 2006-06-29
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公开(公告)号: US20070143880A1公开(公告)日: 2007-06-21
- 发明人: Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao
- 申请人: Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu , Zuo-Yu Zhao
- 申请人地址: US IA Johnston
- 专利权人: PIONEER HI-BRED INTERNATIONAL, INC.
- 当前专利权人: PIONEER HI-BRED INTERNATIONAL, INC.
- 当前专利权人地址: US IA Johnston
- 主分类号: A01H1/00
- IPC分类号: A01H1/00 ; C12N15/82
摘要:
The present invention provides methods and compositions which deliver Agrobacterium via microinjection directly into the embryo sac. At the time of injection, the embryo sac can comprise an egg cell, or alternatively, the embryo sac can be fertilized and comprise either a zygote or an embryo. Once inside the embryo sac, the Agrobacterium harboring a T-DNA having a polynucleotide of interest can express of the polynucleotide of interest in the plant. Further, the Agrobacterium can transfer the T-DNA having the polynucleotide of interest to the plant nucleus to produce a transformed plant. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and any tissue, plant part, and/or plant generated therefrom.