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    Methods for Introducing Into a Plant a Polynucleotide of Interest
    1.
    发明申请
    Methods for Introducing Into a Plant a Polynucleotide of Interest 审中-公开
    向植物引入感兴趣的多核苷酸的方法

    公开(公告)号：US20070143880A1

    公开(公告)日：2007-06-21

    申请号：US11427371

    申请日：2006-06-29

    申请人： Dennis L. Bidney ,  Sheila E. Maddock ,  Xinli E. Wu ,  Zuo-Yu Zhao

    发明人： Dennis L. Bidney ,  Sheila E. Maddock ,  Xinli E. Wu ,  Zuo-Yu Zhao

    IPC分类号： A01H1/00 ,  C12N15/82

    CPC分类号： C12N15/8205 ,  C12N15/8207 ,  C12N15/8213

    摘要： The present invention provides methods and compositions which deliver Agrobacterium via microinjection directly into the embryo sac. At the time of injection, the embryo sac can comprise an egg cell, or alternatively, the embryo sac can be fertilized and comprise either a zygote or an embryo. Once inside the embryo sac, the Agrobacterium harboring a T-DNA having a polynucleotide of interest can express of the polynucleotide of interest in the plant. Further, the Agrobacterium can transfer the T-DNA having the polynucleotide of interest to the plant nucleus to produce a transformed plant. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and any tissue, plant part, and/or plant generated therefrom.

    摘要翻译： 本发明提供了通过显微注射将土壤杆菌直接递送到胚囊中的方法和组合物。 在注射时，胚囊可以包括卵细胞，或者，胚囊可被受精并且包括受精卵或胚胎。 一旦进入胚囊，携带具有感兴趣的多核苷酸的T-DNA的农杆菌可以在植物中表达感兴趣的多核苷酸。 此外，农杆菌可以将具有目标多核苷酸的T-DNA转移到植物核，以产生转化的植物。 感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳的基因组中，以及由其产生的任何组织，植物部分和/或植物。

    Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
    2.
    发明申请
    Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange 审中-公开
    提高位点特异性多核苷酸交换效率的方法和组合

    公开(公告)号：US20070143881A1

    公开(公告)日：2007-06-21

    申请号：US11427947

    申请日：2006-06-30

    申请人： Dennis L. Bidney ,  Sheila E. Maddock ,  Xinli E. Wu

    发明人： Dennis L. Bidney ,  Sheila E. Maddock ,  Xinli E. Wu

    IPC分类号： A01H1/00

    CPC分类号： C12N15/8213 ,  C12N15/8205 ,  C12N15/8207

    摘要： Methods and compositions using a site-specific integration system are combined with methods and compositions which deliver compositions via microinjection directly to the embryo sac of a plant. The methods allow for various components of the site-specific recombination system to be introduced into the cellular environment of the embryo sac a composition comprising at least one component of the site-specific recombination system is injected into an embryo sac, providing improved efficiency of expression, recombination, integration, exchange, excision and/or inversion of a polynucleotide of interest. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and tissues, plant parts, and/or plants produced therefrom. Cells, egg cells, zygotes, embryos, endosperm, tissues, seeds, and/or plants produced by the methods and comprising the polynucleotide(s) of interest are also provided.

    摘要翻译： 使用位点特异性整合系统的方法和组合物与通过显微注射将组合物直接递送至植物胚囊的方法和组合物组合。 所述方法允许将位点特异性重组系统的各种组分引入胚囊的细胞环境中，将包含位点特异性重组系统的至少一个组分的组合物注入胚囊，提供更好的表达效率 ，重组，整合，交换，切除和/或转化感兴趣的多核苷酸。 感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳以及由其产生的组织，植物部分和/或植物的基因组中。 还提供了通过方法产生并包含感兴趣的多核苷酸的细胞，卵细胞，合子，胚胎，胚乳，组织，种子和/或植物。

    Methods for improving monocot transformation
    6.
    发明授权
    Methods for improving monocot transformation 失效
    改善单子叶植物转化的方法

    公开(公告)号：US08404930B2

    公开(公告)日：2013-03-26

    申请号：US12694013

    申请日：2010-01-26

    申请人： Xinli E. Wu ,  Myeong-Je Cho ,  Zuo-Yu Zhao

    发明人： Xinli E. Wu ,  Myeong-Je Cho ,  Zuo-Yu Zhao

    IPC分类号： C12N15/84 ,  A01H4/00

    CPC分类号： C12N15/8205

    摘要： The invention provides improved plant transformation methods. In particular the method provides increased transformation frequency, especially in recalcitrant plants. The method includes various transformation protocols for monocots, such as maize and sorghum, using a combination of media and light conditions to achieve increased efficiency of monocot transformation and increased callus initiation frequencies.

    摘要翻译： 本发明提供改进的植物转化方法。 特别地，该方法提供增加的转化频率，特别是在顽固植物中。 该方法包括用于单子叶植物（例如玉米和高粱）的各种转化方案，其使用培养基和光照条件的组合来实现提高单子叶植物转化的效率和增加的愈伤组织起始频率。

    Method for increasing efficiency of germplasm screening in plant transformation
    7.
    发明授权
    Method for increasing efficiency of germplasm screening in plant transformation 失效
    提高植物转化种质筛选效率的方法

    公开(公告)号：US08389807B2

    公开(公告)日：2013-03-05

    申请号：US12649673

    申请日：2009-12-30

    申请人： Zuo-Yu Zhao ,  Xinli E. Wu ,  Myoeng-Je Cho ,  Deping Xu

    发明人： Zuo-Yu Zhao ,  Xinli E. Wu ,  Myoeng-Je Cho ,  Deping Xu

    IPC分类号： C12N15/84 ,  A01H4/00 ,  A01H1/04

    CPC分类号： C12N15/8209 ,  A01H1/04 ,  C12N15/8201 ,  C12N15/8205

    摘要： A method for increasing efficiency of germplasm screening for transformability may include providing a plurality of lines of plant target tissue to be transformed, characterizing each of the lines to provide characterization data, the characterization data comprises DNA or nucleic acid delivery technique response data and tissue culture response data, eliminating one or more of the plurality of lines based on the characterization data without performing transformation of the plurality of lines, such that a subset of the plurality of lines remains, and performing transformation experiments on the subset of the plurality of lines. The method may also include selecting a DNA or nucleic acid delivery technique protocol and a tissue culture protocol prior to the characterization.

    摘要翻译： 提高种质筛选效率的方法可包括提供要转化的多条植物靶组织，表征各条线以提供特征数据，表征数据包括DNA或核酸递送技术应答数据和组织培养 响应数据，基于所述表征数据消除所述多行中的一个或多个，而不执行所述多条线的变换，使得保留所述多条线的子集，并对所述多条线的所述子集执行变换实验。 该方法还可以包括在表征之前选择DNA或核酸递送技术方案和组织培养方案。