发明申请
US20070184472A1 Method of purifying environmental dna and method of efficiently screening for protein-encoding gene from environmental dna
审中-公开
环境dna的纯化方法和有效筛选环境DNA的蛋白质编码基因的方法
- 专利标题： Method of purifying environmental dna and method of efficiently screening for protein-encoding gene from environmental dna
- 专利标题（中）： 环境dna的纯化方法和有效筛选环境DNA的蛋白质编码基因的方法
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申请号： US11628730申请日： 2005-06-08
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公开(公告)号： US20070184472A1公开(公告)日： 2007-08-09
- 发明人: Masaji Okamoto , Kenichi Tanaka
- 申请人： Masaji Okamoto , Kenichi Tanaka
- 申请人地址： JP Tokyo 105-8419
- 专利权人： TOAGOSEI CO., LTD
- 当前专利权人： TOAGOSEI CO., LTD
- 当前专利权人地址： JP Tokyo 105-8419
- 国际申请： PCT/JP05/10907 WO 20050608
- 主分类号： C40B30/06
- IPC分类号： C40B30/06 ; C40B40/08 ; C07H21/04
摘要：
The method of purifying environmental DNA and the method of screening for a gene encoding a functional protein from environmental DNA are provided. DNA from a environmental sample is purified by recovering DNA from an environmental sample and allowing the thus recovered DNA to be subjected to gel filtration chromatography and ion exchange chromatography. In addition, a display library of DNA derived from an environmental sample is created so that screening is carried out in terms of the interaction of the above display library with ligands. Thus, screening of protein-encoding genes can be carried out.
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