A method of producing an antibody recognizing a target fatty acid alcohol ester by immunizing an animal with a carrier protein conjugated with a derivative of the target fatty acid alcohol ester. A method of estimating alcohol consumption of an individual by quantitating levels of molecules which bind to antibodies produced with a derivative of a target fatty acid alcohol ester conjugated to a carrier protein in a biological sample obtained from the individual. A kit for estimating alcohol consumption of an individual including means for quantitating levels of molecules which bind to antibodies produced with a derivative of a target fatty acid alcohol ester conjugated to a carrier protein in a biological sample obtained from the individual.