发明申请
- 专利标题： Method for purifying plasmid DNA
- 专利标题（中）： 纯化质粒DNA的方法
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申请号： US11582427申请日： 2006-10-18
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公开(公告)号： US20070213289A1公开(公告)日： 2007-09-13
- 发明人: Francis Blanche , Michel Couder , Nicolas Maestrali , Thierry Guillemin , David Gaillac
- 申请人： Francis Blanche , Michel Couder , Nicolas Maestrali , Thierry Guillemin , David Gaillac
- 申请人地址： FR Vitry Sur Seine
- 专利权人： CENTELION
- 当前专利权人： CENTELION
- 当前专利权人地址： FR Vitry Sur Seine
- 主分类号： A61K48/00
- IPC分类号： A61K48/00 ; C12P19/34
摘要：
This invention provides a process for the continuous alkaline lysis of a bacterial suspension in order to harvest pDNA. It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatogragphy, and hydrophobic interaction chromatography. These optional purification steps can be combined with the continuous lysis in order to produce a highly purified pDNA product substantially free of gDNA, RNA, protein, endotoxin, and other contaminants.
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