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公开(公告)号：US06518062B1
公开(公告)日：2003-02-11
申请号：US09125576
申请日：1998-09-10
申请人： Francis Blanche , Béatrice Cameron , Michel Couder , Joël Crouzet
发明人： Francis Blanche , Béatrice Cameron , Michel Couder , Joël Crouzet
IPC分类号： C12N1500
CPC分类号： A61K38/45 , A61K48/00 , C12N9/1211 , A61K2300/00
摘要： Enzyme combinations useful for destroying cells, particularly proliferative cells, are disclosed. In particular, a combination of the following enzymes has been found to enhance the toxicity of nucleoside analogues to proliferating cells: an enzyme that phosphorylates the non-toxic nucleoside analogue to generate a monophosphate analogue, an enzyme that phosphorylates the monophosphate analogue to generate a diphosphate analogue and an enzyme that phosphorylates the diphosphate analogue to generate a toxic triphosphate analogue. Vectors enabling the intracellular expression and transfer of the enzyme combinations, as well as their therapeutic use, particularly in anti-cancer gene therapy, are also disclosed.
摘要翻译： 公开了可用于破坏细胞，特别是增殖细胞的酶组合。 特别地，已经发现以下酶的组合增强核苷类似物对增殖细胞的毒性：使无毒核苷类似物磷酸化以产生单磷酸酯类似物的酶，磷酸化单磷酸酯类似物以产生二磷酸 类似物和使磷酸化类似物产生有毒的三磷酸酯类似物的酶。 还公开了能够进行酶组合的细胞内表达和转移的载体及其治疗用途，特别是在抗癌基因治疗中。
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公开(公告)号：US20070213289A1
公开(公告)日：2007-09-13
申请号：US11582427
申请日：2006-10-18
CPC分类号： A61K48/0091 , C02F2303/06 , C12N15/101 , C12P19/34
摘要： This invention provides a process for the continuous alkaline lysis of a bacterial suspension in order to harvest pDNA. It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatogragphy, and hydrophobic interaction chromatography. These optional purification steps can be combined with the continuous lysis in order to produce a highly purified pDNA product substantially free of gDNA, RNA, protein, endotoxin, and other contaminants.
摘要翻译： 本发明提供了一种用于连续碱裂解细菌悬浮液以便收获pDNA的方法。 它进一步提供可选的附加纯化步骤，包括裂解物过滤，阴离子交换层析，三重亲和层析和疏水相互作用层析。 这些任选的纯化步骤可以与连续裂解组合以产生基本上不含gDNA，RNA，蛋白质，内毒素和其他污染物的高度纯化的pDNA产物。
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3.发明授权Variants of thymidine kinase, nucleic acids encoding them, and methods of using them 失效胸苷激酶的变体，编码它们的核酸，以及使用它们的方法公开(公告)号：US06207150B1
公开(公告)日：2001-03-27
申请号：US09125099
申请日：1998-08-06
申请人： Joël Crouzet , Francis Blanche , Michel Couder , Béatrice Cameron
发明人： Joël Crouzet , Francis Blanche , Michel Couder , Béatrice Cameron
IPC分类号： A61K3851
CPC分类号： C12N9/1211 , A01K2217/05 , A61K38/00
摘要： The present invention relates to a nucleic acid sequence characterized in that it is derived from the wild nucleic acid sequence coding for a thymidine kinase, said nucleic acid sequence having at least one mutation in the region corresponding to the ATP binding site and conveniently a second mutation in the N-terminal region and/or C-terminal region. It also relates to variants of the wild thymidine kinase and their use in genic therapy.
摘要翻译： 本发明涉及一种核酸序列，其特征在于其衍生自编码胸苷激酶的野生型核酸序列，所述核酸序列在对应于ATP结合位点的区域中具有至少一个突变，并且方便地具有第二突变 在N-末端区域和/或C-末端区域。 它还涉及野生胸苷激酶的变体及其在基因治疗中的用途。
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公开(公告)号：US20070111221A1
公开(公告)日：2007-05-17
申请号：US11384191
申请日：2006-03-17
CPC分类号： A61K48/0091 , C12M47/06 , C12N1/06 , C12N15/1003
摘要： This invention provides a process for the continuous alkaline lysis of a bacterial suspension in order to harvest pDNA. It further provides for optional additional purification steps, including lysate filtration, anion exchange chromatography, triplex affinity chromatography, and hydrophobic interaction chromatography. These optional purification steps can be combined with the continuous lysis in order to produce a highly purified pDNA product substantially free of gDNA, RNA, protein, endotoxin, and other contaminants.
摘要翻译： 本发明提供了一种用于连续碱裂解细菌悬浮液以便收获pDNA的方法。 它进一步提供可选的附加纯化步骤，包括裂解物过滤，阴离子交换层析，三重亲和层析和疏水相互作用层析。 这些任选的纯化步骤可以与连续裂解组合以产生基本上不含gDNA，RNA，蛋白质，内毒素和其他污染物的高度纯化的pDNA产物。
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