发明申请
- 专利标题: Side Chain Deuterated Amino Acids Methods Of Use
- 专利标题(中): 侧链氘代氨基酸使用方法
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申请号: US10574967申请日: 2004-10-07
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公开(公告)号: US20070275442A1公开(公告)日: 2007-11-29
- 发明人: Jonathan Brown , Steven Homans , Michael Chaykovsky , Jenny Murray
- 申请人: Jonathan Brown , Steven Homans , Michael Chaykovsky , Jenny Murray
- 申请人地址: US MD Columbia 21046
- 专利权人: ProSpect Pharma
- 当前专利权人: ProSpect Pharma
- 当前专利权人地址: US MD Columbia 21046
- 国际申请: PCT/US04/32941 WO 20041007
- 主分类号: G01N33/53
- IPC分类号: G01N33/53 ; C07C211/01 ; G01N24/08 ; C12P21/06
摘要:
Protein structural determination using NMR techniques is improved through use of proteins in which one or more amino acids in the peptidic sequence are isotopically enriched in the sidechain with 2H and are isotopically enriched on the backbone with 13C, 15N, 2H or any combination thereof. This invention provides amino acids isotopically enriched as above, which can be used to synthesize isotopically labeled proteins and peptides for protein structural determinations by NMR, and methods for their synthesis. Other embodiments of the invention include peptidic molecules, media for peptidic molecule expression, methods of making isotopically labeled peptidic molecules and methods of determining structural information of a peptidic molecule.