发明申请
- 专利标题: Method of Separating Pancreatic Islet
- 专利标题(中): 分离胰岛的方法
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申请号: US11793679申请日: 2005-12-22
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公开(公告)号: US20080038823A1公开(公告)日: 2008-02-14
- 发明人: Shinichi Matsumoto , Hiromi Wada , Hirofumi Noguchi
- 申请人: Shinichi Matsumoto , Hiromi Wada , Hirofumi Noguchi
- 申请人地址: JP Kyoto-shi 606-8501
- 专利权人: KYOTO UNIVERSITY
- 当前专利权人: KYOTO UNIVERSITY
- 当前专利权人地址: JP Kyoto-shi 606-8501
- 优先权: JP2004-370732 20041222; JP2005-211228 20050721
- 国际申请: PCT/JP05/23602 WO 20051222
- 主分类号: C12N5/02
- IPC分类号: C12N5/02
摘要:
The present invention provides a pancreatic islet isolation method comprising the steps of (1) injecting a protection solution containing a protease inhibitor into the pancreatic duct of an procured pancreas; (3) digesting the pancreas into which the protection solution has been injected; and (4) purifying the digested pancreatic tissue using a purification solution containing a density gradient reagent. The present invention also provides a protection solution for injection into the pancreatic duct, a pancreas preservation solution for the two-layer method, and an islet purification solution.
公开/授权文献
- US08288085B2 Method of separating pancreatic islet 公开/授权日:2012-10-16
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