发明申请
US20080171348A1 GFP-SNAP25 Fluorescence Release Assay for Botulinum Toxin Protease Activity
审中-公开
GFP-SNAP25荧光释放测定肉毒杆菌毒素蛋白酶活性
- 专利标题： GFP-SNAP25 Fluorescence Release Assay for Botulinum Toxin Protease Activity
- 专利标题（中）： GFP-SNAP25荧光释放测定肉毒杆菌毒素蛋白酶活性
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申请号： US12019188申请日： 2008-01-24
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公开(公告)号： US20080171348A1公开(公告)日： 2008-07-17
- 发明人: Lance E. Steward , Marcella A. Gilmore , Kei R. Aoki
- 申请人： Lance E. Steward , Marcella A. Gilmore , Kei R. Aoki
- 专利权人： Allergan, Inc.
- 当前专利权人： Allergan, Inc.
- 主分类号： C12Q1/37
- IPC分类号： C12Q1/37
摘要：
The present invention provides a nucleic acid molecule which contains a nucleotide sequence encoding a SNAP-25 substrate which includes (i) a green fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a portion of SNAP-25 that includes a BoNT/A, BoNT/C1 or BoNT/E recognition sequence containing a cleavage site, where the cleavage site intervenes between the green fluorescent protein and the first partner of the affinity couple. Further provided herein is a nucleic acid molecule which contains a nucleotide sequence encoding a tagged toxin substrate which includes (i) a fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a clostridial toxin recognition sequence containing a cleavage site, where the cleavage site intervenes between the fluorescent protein and the first partner of the affinity couple.
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