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    GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
    3.
    发明授权
    GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity 有权
    GFP-SNAP25荧光释放测定肉毒杆菌神经毒素蛋白酶活性

    公开(公告)号：US07374896B2

    公开(公告)日：2008-05-20

    申请号：US10917844

    申请日：2004-08-13

    申请人： Lance E. Steward ,  Marcella A. Gilmore ,  Kei R. Aoki

    发明人： Lance E. Steward ,  Marcella A. Gilmore ,  Kei R. Aoki

    IPC分类号： G01N33/554 ,  G01N33/53 ,  C12Q1/37 ,  C12Q1/06 ,  C12P21/06 ,  C12N1/20 ,  C12N15/74

    CPC分类号： C07K14/705 ,  C07K14/001 ,  C07K14/435 ,  C07K14/43595 ,  C07K2319/00 ,  C12N9/52 ,  C12Q1/37 ,  C12Y304/24069 ,  G01N33/56911 ,  G01N2333/33

    摘要： The present invention provides a nucleic acid molecule which contains a nucleotide sequence encoding a SNAP-25 substrate which includes (i) a green fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a portion of SNAP-25 that includes a BoNT/A, BoNT/C1 or BoNT/E recognition sequence containing a cleavage site, where the cleavage site intervenes between the green fluorescent protein and the first partner of the affinity couple. Further provided herein is a nucleic acid molecule which contains a nucleotide sequence encoding a tagged toxin substrate which includes (i) a fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a clostridial toxin recognition sequence containing a cleavage site, where the cleavage site intervenes between the fluorescent protein and the first partner of the affinity couple.

    摘要翻译： 本发明提供了含有编码SNAP-25底物的核苷酸序列的核酸分子，其包括（i）绿色荧光蛋白; （ii）亲和夫妇的第一个伴侣; 和（iii）SNAP-25的一部分，其包括含有切割位点的BoNT / A，BoNT / C1或BoNT / E识别序列，其中所述切割位点介于所述绿色荧光蛋白和所述亲和力对的第一个伴侣之间。 本文还提供了含有编码标记的毒素底物的核苷酸序列的核酸分子，其包括（i）荧光蛋白; （ii）亲和夫妇的第一个伴侣; 和（iii）含有切割位点的梭菌毒素识别序列，其中所述切割位点介入所述荧光蛋白和所述亲和力对的第一个配偶体之间。

    GFP-SNAP25 Fluorescence Release Assay for Botulinum Toxin Protease Activity
    6.
    发明申请
    GFP-SNAP25 Fluorescence Release Assay for Botulinum Toxin Protease Activity 审中-公开
    GFP-SNAP25荧光释放测定肉毒杆菌毒素蛋白酶活性

    公开(公告)号：US20080171348A1

    公开(公告)日：2008-07-17

    申请号：US12019188

    申请日：2008-01-24

    申请人： Lance E. Steward ,  Marcella A. Gilmore ,  Kei R. Aoki

    发明人： Lance E. Steward ,  Marcella A. Gilmore ,  Kei R. Aoki

    IPC分类号： C12Q1/37

    CPC分类号： C07K14/705 ,  C07K14/001 ,  C07K14/435 ,  C07K14/43595 ,  C07K2319/00 ,  C12N9/52 ,  C12Q1/37 ,  C12Y304/24069 ,  G01N33/56911 ,  G01N2333/33

    摘要： The present invention provides a nucleic acid molecule which contains a nucleotide sequence encoding a SNAP-25 substrate which includes (i) a green fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a portion of SNAP-25 that includes a BoNT/A, BoNT/C1 or BoNT/E recognition sequence containing a cleavage site, where the cleavage site intervenes between the green fluorescent protein and the first partner of the affinity couple. Further provided herein is a nucleic acid molecule which contains a nucleotide sequence encoding a tagged toxin substrate which includes (i) a fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a clostridial toxin recognition sequence containing a cleavage site, where the cleavage site intervenes between the fluorescent protein and the first partner of the affinity couple.

    摘要翻译： 本发明提供了含有编码SNAP-25底物的核苷酸序列的核酸分子，其包括（i）绿色荧光蛋白; （ii）亲和夫妇的第一个伴侣; 和（iii）SNAP-25的一部分，其包括含有切割位点的BoNT / A，BoNT / C1或BoNT / E识别序列，其中所述切割位点介于所述绿色荧光蛋白和所述亲和力对的第一个伴侣之间。 本文还提供了含有编码标记的毒素底物的核苷酸序列的核酸分子，其包括（i）荧光蛋白; （ii）亲和夫妇的第一个伴侣; 和（iii）含有切割位点的梭菌毒素识别序列，其中所述切割位点介入所述荧光蛋白和所述亲和力对的第一个配偶体之间。

    Agent and methods for treating pain
    7.
    发明授权
    Agent and methods for treating pain 失效
    治疗疼痛的药剂和方法

    公开(公告)号：US06787517B1

    公开(公告)日：2004-09-07

    申请号：US09751053

    申请日：2000-12-29

    申请人： Daniel W. Gil ,  Kei R. Aoki

    发明人： Daniel W. Gil ,  Kei R. Aoki

    IPC分类号： A01N6100

    CPC分类号： A61K38/56 ,  A61K38/168 ,  A61K38/4893 ,  A61K47/54 ,  A61K47/545 ,  A61K47/6415 ,  A61K47/6829 ,  A61K47/6843 ,  C07K16/18

    摘要： Agents for treating pain, methods for producing the agents and methods for treating pain by administration to a patient of a therapeutically effective amount of the agent are disclosed. The agent may include a clostridial neurotoxin, a fragment or a derivative thereof, attached to a targeting component, wherein the targeting component is selected from a group consisting of compounds which selectively binds at the alpha-2B or alpha-2B/alpha-2C adrenergic receptor subtype(s) as compared to other binding sites, for example, the alpha-2A adrenergic receptor subtype.

    摘要翻译： 公开了用于治疗疼痛的药剂，用于制备药剂的方法和通过向患者施用治疗有效量的药剂来治疗疼痛的方法。 所述试剂可以包含连接到靶向组分的梭菌神经毒素，其片段或衍生物，其中所述靶向组分选自由α-2B或α-2B /α-2C肾上腺素能选择性结合的化合物 受体亚型与其他结合位点相比，例如α-2A肾上腺素能受体亚型。

    Methods for treating muscle injuries
    8.
    发明授权
    Methods for treating muscle injuries 有权
    治疗肌肉损伤的方法

    公开(公告)号：US06423319B1

    公开(公告)日：2002-07-23

    申请号：US09678189

    申请日：2000-10-04

    申请人： Gregory F Brooks ,  Kei R. Aoki

    发明人： Gregory F Brooks ,  Kei R. Aoki

    IPC分类号： A61K3908

    CPC分类号： A61K38/4893 ,  A61K39/08 ,  A61K2039/54

    摘要： Methods for treating an injured muscle by local administration of a neurotoxin, such as a botulinum toxin, to promote healing and/or to reduce the pain associated with an injured muscle.

    摘要翻译： 通过局部施用神经毒素（例如肉毒杆菌毒素）来治疗受伤的肌肉以促进愈合和/或减少与受损肌肉相关的疼痛的方法。

    GFP-SNAP25 fluorescence release assay for botulinum toxin protease activity
    9.
    发明授权
    GFP-SNAP25 fluorescence release assay for botulinum toxin protease activity 有权
    GFP-SNAP25荧光释放测定用于肉毒杆菌毒素蛋白酶活性

    公开(公告)号：US07838260B2

    公开(公告)日：2010-11-23

    申请号：US12019120

    申请日：2008-01-24

    申请人： Lance E. Steward ,  Marcella A. Gilmore ,  Kei R. Aoki

    发明人： Lance E. Steward ,  Marcella A. Gilmore ,  Kei R. Aoki

    IPC分类号： G01N33/554 ,  G01N33/53 ,  G01N33/569 ,  C12Q1/37 ,  C12Q1/06

    CPC分类号： C07K14/705 ,  C07K14/001 ,  C07K14/435 ,  C07K14/43595 ,  C07K2319/00 ,  C12N9/52 ,  C12Q1/37 ,  C12Y304/24069 ,  G01N33/56911 ,  G01N2333/33

    摘要： The present invention provides a nucleic acid molecule which contains a nucleotide sequence encoding a SNAP-25 substrate which includes (i) a green fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a portion of SNAP-25 that includes a BoNT/A, BoNT/C1 or BoNT/E recognition sequence containing a cleavage site, where the cleavage site intervenes between the green fluorescent protein and the first partner of the affinity couple. Further provided herein is a nucleic acid molecule which contains a nucleotide sequence encoding a tagged toxin substrate which includes (i) a fluorescent protein; (ii) a first partner of an affinity couple; and (iii) a clostridial toxin recognition sequence containing a cleavage site, where the cleavage site intervenes between the fluorescent protein and the first partner of the affinity couple.

    摘要翻译： 本发明提供了含有编码SNAP-25底物的核苷酸序列的核酸分子，其包括（i）绿色荧光蛋白; （ii）亲和夫妇的第一个伴侣; 和（iii）SNAP-25的一部分，其包括含有切割位点的BoNT / A，BoNT / C1或BoNT / E识别序列，其中所述切割位点介于所述绿色荧光蛋白和所述亲和力对的第一个伴侣之间。 本文还提供了含有编码标记的毒素底物的核苷酸序列的核酸分子，其包括（i）荧光蛋白; （ii）亲和夫妇的第一个伴侣; 和（iii）含有切割位点的梭菌毒素识别序列，其中所述切割位点介入所述荧光蛋白和所述亲和力对的第一个配偶体之间。