- 专利标题: Method and device for electroporation of biological cells
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申请号: US12072016申请日: 2008-02-22
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公开(公告)号: US20080220491A1公开(公告)日: 2008-09-11
- 发明人: Ulrich Zimmermann , Vladimir Sukhorukov , Kurt Lucas
- 申请人: Ulrich Zimmermann , Vladimir Sukhorukov , Kurt Lucas
- 专利权人: EPPENDORF AG
- 当前专利权人: EPPENDORF AG
- 优先权: DE10202094.9 20020121
- 主分类号: C12N13/00
- IPC分类号: C12N13/00 ; C12M1/34
摘要:
Described are methods for electrical treatment of biological cells, in particular using electrical field pulses, involving the steps: arrangement of the cells (1) on apertures (2) of a solid planar carrier element (3) which divides a measuring chamber (10) into two compartments (11, 12); and temporary formation of an electrical treatment field which permeates the cells, wherein an alternating-current impedance measurement takes place on the carrier element (3), and from the result of the alternating-current impedance measurement, a degree of coverage of the carrier element and/or healing of the cells after electrical treatment are/is acquired. Also described are devices for implementing the methods.
公开/授权文献
- US08465955B2 Method for electroporation of biological cells 公开/授权日:2013-06-18
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