It is intended to provide a supersensitive, quick and accurate method for biochemical analysis. The method includes: adding magnetic fine particles into a solution containing a target substance, whereby a first substance for detection immobilized on the magnetic fine particle is bound to the target substance, while aggregating the magnetic fine particles so as to form an aggregate in the solution; next binding the target substance bound with the magnetic fine particle constituting the aggregate to a second substance for detection on a magnetic sensor layer so as to immobilize the aggregate onto the surface of the magnetic sensor layer; and measuring a magnetic stray field of this aggregate using a magnetic sensor.