An system and method provide the ability to image a biological sample. A sample is embed to a support matrix that is compatible with an aqueous nature of the sample. A vibrating tissue sectioning system is coupled to a microscope and is used to remove a region of the sample without moving the sample. The sectioning of the sample occurs under a surface of an aqueous buffer in a basin. A positioning system enables the microscope to image adjacent sub-regions of the sample. The microscope image multiple sections of the sample in adjacent subregions using the vibrating tissue sectioning system and the positioning system.