System and method for detection and tracking of targets, which in a preferred embodiment is based on the use of fractional Fourier transformation of time-domain signals to compute projections of the auto and cross ambiguity functions along arbitrary line segments. The efficient computational algorithms of the preferred embodiment are used to detect the position and estimate the velocity of signals, such as those encountered by active or passive sensor systems. Various applications of the proposed algorithm in the analysis of time-frequency domain signals are also disclosed.