发明申请
- 专利标题: CONSTITUTIVELY TRANSLOCATING CELL LINE
- 专利标题(中): 一般转录细胞系
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申请号: US11936939申请日: 2007-11-08
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公开(公告)号: US20090082410A1公开(公告)日: 2009-03-26
- 发明人: Robert H. Oakley , Christine C. Hudson
- 申请人: Robert H. Oakley , Christine C. Hudson
- 申请人地址: US CA Sunnyvale
- 专利权人: Molecular Devices Corporation
- 当前专利权人: Molecular Devices Corporation
- 当前专利权人地址: US CA Sunnyvale
- 优先权: FR0301914 20030218
- 主分类号: A61K31/4164
- IPC分类号: A61K31/4164 ; C12Q1/02 ; G01N33/53 ; C07K14/00 ; A61P3/00 ; A61P9/00 ; C07H21/00 ; C12N15/63 ; C12N1/00
摘要:
The present invention relates to agonist-independent methods of screening for compounds that alter GPCR desensitization. Included in the present invention are cell lines containing GRKs, in which GPCRs are desensitized in the absence of agonist; the GRKs may be modified. The present invention relates to methods to determine if a GPCR is expressed at the plasma membrane, and if the GPCR has an affinity for arrestin. Modified GPCRs which have increased arrestin affinity are included in the present invention. These modified GPCRs are useful in methods to screen for compounds that alter desensitization, including both the agonist-independent methods and agonist-dependent methods described herein.
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