A display panel and a color filter substrate thereof are provided. The display panel includes a first substrate, an alignment structure set, a second substrate, a pixel electrode, and a liquid crystal layer. The alignment structure set includes a first and a second alignment units disposed on the upper electrode. The first alignment unit is different from the second alignment unit. The pixel electrode is formed on the second substrate and includes a first electrode and a second lower electrode opposite to the first and second alignment units, respectively. A color filter may be disposed between the first substrate and the alignment structure layer to form a color filter substrate.