发明申请
US20090203008A1 Rapid method to determine inhibitor sensitivity of NS3/4A protease sequences cloned from clinical samples
审中-公开
确定从临床样品克隆的NS3 / 4A蛋白酶序列的抑制剂敏感性的快速方法
- 专利标题： Rapid method to determine inhibitor sensitivity of NS3/4A protease sequences cloned from clinical samples
- 专利标题（中）： 确定从临床样品克隆的NS3 / 4A蛋白酶序列的抑制剂敏感性的快速方法
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申请号： US12308010申请日： 2007-06-04
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公开(公告)号： US20090203008A1公开(公告)日： 2009-08-13
- 发明人: Steven W. Ludmerer , Donald J. Graham , David B. Olsen
- 申请人： Steven W. Ludmerer , Donald J. Graham , David B. Olsen
- 国际申请： PCT/US07/13154 WO 20070604
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68
摘要：
A method for measuring HCV NS3/4A activity from a HCV NS3/4A sequence, comprising obtaining and cloning the sequence into a mammalian expression vector, transiently transfecting a mammalian cell with the vector, which includes a reporter construct encoding a HCV NS3/4A cleavage site fused to a detectable label, measuring signal production from the label resulting from cleavage at the cleavage site, and measuring effects on signal production by addition of a test compound.