A driving method adapted to driving an optical touch panel is provided. The optical touch panel is disposed on a backlight module and has a plurality of touch sensing elements. The touch sensing elements have a light-shading sensing mode and a light-reflecting sensing mode. The driving method includes sensing an intensity of an ambient light. When the intensity of the ambient light is greater than a reference, the touch sensing elements operate in the light-shading sensing mode. Alternately, when the intensity of the ambient light is smaller than the reference, the touch sensing elements operate in the light-reflecting sensing mode. The abovementioned driving method is help for the optical touch panel to accurately sensing in various conditions of different ambient lights such that the usage of the optical touch panel is more convenient.