发明申请
US20090220942A1 ACTIVATED SPLIT-POLYPEPTIDES AND METHODS FOR THEIR PRODUCTION AND USE
审中-公开
活性分离多肽及其生产和使用方法
- 专利标题： ACTIVATED SPLIT-POLYPEPTIDES AND METHODS FOR THEIR PRODUCTION AND USE
- 专利标题（中）： 活性分离多肽及其生产和使用方法
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申请号： US12091709申请日： 2006-10-27
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公开(公告)号： US20090220942A1公开(公告)日： 2009-09-03
- 发明人: Natalia Broude , Charles R. Cantor , Vadim V. Demidov
- 申请人： Natalia Broude , Charles R. Cantor , Vadim V. Demidov
- 国际申请： PCT/US06/42299 WO 20061027
- 主分类号： C12Q1/70
- IPC分类号： C12Q1/70 ; C12Q1/68 ; C07K14/00 ; C12P21/00
摘要：
The present invention relates to a method to produce activated split-polypeptide fragments that on reconstitution immediately forms an active protein. The method relate to real-time protein complementation. Also encompassed in the invention is a method to split and produce split-fluorescent proteins in an active state which produce a fluorescent signal immediately on reconstitution. The present application also provides methods to detect nucleic acids; non-nucleic acid analytes and nucleic acid hybridization in real-time using the novel activated split-polypeptide fragments of the invention.
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