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公开(公告)号：US06265166B1
公开(公告)日：2001-07-24
申请号：US09302201
申请日：1999-04-29
IPC分类号： C12Q168
CPC分类号： C12Q1/6839 , C12Q1/6832 , C12Q2525/307 , C12Q2525/107 , C12Q2535/101 , C12Q2535/119
摘要： A stable complex, we refer to as a PD-Loop, between double stranded nucleic acid and a nucleobase polymer is assembled with the aid of strand invading peptide nucleic acid (PNA). The PD-Loop can be used in the detection, analysis, quantitation and even in the affinity capture of the duplex nucleic acid. Alternatively, the PD-Loop can be used to initiate polymerase extension of a primer to thereby facilitate sequencing of the double stranded nucleic acid even in the presence of large excesses of unrelated double stranded nucleic acid. As an additional feature, the PD-Loop can also be used to generate a construct comprised of a double stranded nucleic acid through which is threaded a single stranded closed circular nucleic acid wherein the closed circular nucleic acid can be used in a signal amplification methodology.
摘要翻译： 借助于链入侵肽核酸（PNA），将双链核酸和核碱基聚合物之间的一个稳定的复合物称为PD-环。 PD-Loop可用于双相核酸的检测，分析，定量甚至亲和力捕获。 或者，PD-Loop可用于启动引物的聚合酶延伸，从而即使在大量过量的不相关双链核酸的存在下也促进双链核酸的测序。 作为另外的特征，PD-Loop还可以用于产生由双链核酸组成的构建物，通过该双链核酸连接单链闭环的核酸，其中封闭的环状核酸可以用于信号放大方法。
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公开(公告)号：US20090220942A1
公开(公告)日：2009-09-03
申请号：US12091709
申请日：2006-10-27
CPC分类号： C12Q1/6883
摘要： The present invention relates to a method to produce activated split-polypeptide fragments that on reconstitution immediately forms an active protein. The method relate to real-time protein complementation. Also encompassed in the invention is a method to split and produce split-fluorescent proteins in an active state which produce a fluorescent signal immediately on reconstitution. The present application also provides methods to detect nucleic acids; non-nucleic acid analytes and nucleic acid hybridization in real-time using the novel activated split-polypeptide fragments of the invention.
摘要翻译： 本发明涉及一种产生活化的裂多肽片段的方法，其在重构时立即形成活性蛋白质。 该方法涉及实时蛋白质互补。 本发明还包括分裂和产生活性状态的分裂荧光蛋白的方法，其在重建时立即产生荧光信号。 本申请还提供了检测核酸的方法; 非核酸分析物和核酸杂交，使用本发明的新型活化的裂解多肽片段。
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3.发明申请公开(公告)号：US20090029370A1
公开(公告)日：2009-01-29
申请号：US12091682
申请日：2006-10-27
CPC分类号： C12Q1/6816 , C12Q1/682 , C12Q2563/107 , C12Q2561/107 , C12Q2522/101
摘要： The present invention relates to a method to detect nucleic acid molecules, such as RNA molecules in vivo using real time protein complementation methods. The invention further relates to methods for detecting nucleic acids, for example RNA in real-time in living cells with a high sensitivity, using a novel split biomolecular conjugate of the invention.
摘要翻译： 本发明涉及使用实时蛋白质互补方法在体内检测核酸分子例如RNA分子的方法。 本发明还涉及使用本发明的新的分裂生物分子缀合物来检测核酸，例如用活性细胞实时检测高灵敏度的核酸的方法。
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公开(公告)号：US06596486B2
公开(公告)日：2003-07-22
申请号：US09758735
申请日：2001-01-11
申请人： Maxim D. Frank-Kamenetskii , Nikolay O. Bukanov , Vadim V. Demidov , Heiko Kuhn , Natalia E. Broude
发明人： Maxim D. Frank-Kamenetskii , Nikolay O. Bukanov , Vadim V. Demidov , Heiko Kuhn , Natalia E. Broude
IPC分类号： C12Q168
CPC分类号： C12Q1/6839 , C12Q1/6832 , C12Q2525/307 , C12Q2525/107 , C12Q2535/101 , C12Q2535/119
摘要： A stable complex, we refer to as a PD-Loop, between double stranded nucleic acid and a nucleobase polymer is assembled with the aid of strand invading peptide nucleic acid (PNA). The PD-Loop can be used in the detection, analysis, quantitation and even in the affinity capture of the duplex nucleic acid. Alternatively, the PD-Loop can be used to initiate polymerase extension of a primer to thereby facilitate sequencing of the double stranded nucleic acid even in the presence of large excesses of unrelated double stranded nucleic acid. As an additional feature, the PD-Loop can also be used to generate a construct comprised of a double stranded nucleic acid through which is threaded a single stranded dosed circular nucleic acid wherein the closed circular nucleic acid can be used in a signal amplification methodology.
摘要翻译： 借助于链入侵肽核酸（PNA），将双链核酸和核碱基聚合物之间的一个稳定的复合物称为PD-环。 PD-Loop可用于双相核酸的检测，分析，定量甚至亲和力捕获。 或者，PD-Loop可用于启动引物的聚合酶延伸，从而即使在大量过量的不相关双链核酸的存在下也促进双链核酸的测序。 作为另外的特征，PD-Loop还可以用于产生由双链核酸组成的构建体，通过该双链核酸连接单链计数的环状核酸，其中封闭的环状核酸可以用于信号放大方法。
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公开(公告)号：US06004750A
公开(公告)日：1999-12-21
申请号：US905567
申请日：1997-08-04
IPC分类号： C12N15/113 , C12Q1/68 , C07H21/04 , C12N9/00 , C12P21/00
CPC分类号： C12N15/113 , C12Q1/683 , C12Q1/6839 , C12N2310/152 , C12N2310/3181 , C12N2310/3183 , C12N2310/33 , C12N2310/3513
摘要： The invention relates to nucleic acid clamps and methods for using nucleic acid clamps, for example, to inhibit gene expression or cleavage, or to specifically cleave a target nucleic acid. Nucleic acid clamps are molecular devices which can bind nucleic acids with affinity and specificity and have a recognition sequence as small as seven bases. Nucleic acid clamps can be used to modify the effective recognition sequence of restriction endonucleases, reducing the frequency and enhancing the length of the recognition sequence, but without diminishing specificity. The invention also relates to methods for the use of nucleic acid clamps for the treatment of disorders involving abnormal gene expression.
摘要翻译： 本发明涉及核酸钳位和使用核酸钳夹例如抑制基因表达或切割或特异性切割靶核酸的方法。 核酸钳位是可以以亲和力和特异性结合核酸并具有小至七个碱基的识别序列的分子装置。 核酸钳可用于修饰限制性内切核酸酶的有效识别序列，降低识别序列的频率和增加长度，但不会降低特异性。 本发明还涉及使用核酸钳用于治疗涉及异常基因表达的病症的方法。
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