发明申请
- 专利标题: Plant transformation with in vivo assembly of a sequence of interest
- 专利标题(中): 植物转化与感兴趣的序列的体内组装
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申请号: US10544135申请日: 2004-01-30
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公开(公告)号: US20090265814A1公开(公告)日: 2009-10-22
- 发明人: Anatoly Giritch , Serik Eliby , Sylvestre Marillonnet , Victor Klimyuk , Yuri Gleba
- 申请人: Anatoly Giritch , Serik Eliby , Sylvestre Marillonnet , Victor Klimyuk , Yuri Gleba
- 申请人地址: DE Munchen
- 专利权人: Icon Genetics AG
- 当前专利权人: Icon Genetics AG
- 当前专利权人地址: DE Munchen
- 优先权: DE10303937.6 20030131
- 国际申请: PCT/EP2004/000892 WO 20040130
- 主分类号: C12N15/87
- IPC分类号: C12N15/87 ; C12N5/04 ; C12Q1/68 ; A01H5/00 ; C40B40/02
摘要:
A process of producing transgenic plants or plant cells stably transformed on a chromosome with a DNA sequence of interest and capable of expressing a function of interest from said DNA sequence of interest, said process comprising (a) providing plant cells or plants with at least two different vectors, whereby (i) said at least two different vectors are adapted to recombine with each other by site-specific recombination in said plant cells for producing a non-replicating recombination product containing said DNA sequence of interest, (ii) said at least two different vectors are adapted for integrating said DNA sequence of interest into said chromosome, (iii) said DNA sequence of interest contains sequence portions from at least two of said at least two different vectors, said sequence portions being necessary for expressing said function of interest from said DNA sequence of interest; and (b) selecting plants or plant cells expressing said function of interest.
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