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1.发明授权Process of generating transplastomic plants or plant cells devoid of a selection marker 失效产生没有选择标记的转基因植物或植物细胞的过程公开(公告)号：US07371923B2
公开(公告)日：2008-05-13
申请号：US10482549
申请日：2002-04-30
申请人： Hans-Ulrich Koop , Stefan Mühlbauer , Sebastian Klaus , Christian Eibl , Fong-Chin Huang , Timothy J. Golds
发明人： Hans-Ulrich Koop , Stefan Mühlbauer , Sebastian Klaus , Christian Eibl , Fong-Chin Huang , Timothy J. Golds
IPC分类号： C12N15/82
CPC分类号： C12N15/8212 , C12N15/821 , C12N15/8214 , C12N15/8257
摘要： A process of generating transgenic plants or plant cells transformed on their plastome and being devoid of a selection marker, comprising the following steps: (a) transforming plastids of a plant or plant cell with a DNA comprising: (i) a nucleotide sequence conferring replication of said DNA in a plant cell, (ii) at least one sequence of interest, (iii) sequences flanking said at least one sequence of interest necessary for stable integration of said at least one sequence of interest into the plastid genome, and (iv) a selection marker outside of said sequences flanking said sequence(s) of interest; (b) allowing integration of said at least one sequence of interest into the plastome in the presence of selective pressure; (c) allowing loss of said selection marker sequence by releasing selective pressure; and (d) recovering cells and/or plants being genetically transformed on their plastomes and being devoid of said selection marker.
摘要翻译： 一种产生转化的植物或植物细胞的方法，所述转基因植物或植物细胞在其塑料体上转化并且没有选择标记，包括以下步骤：（a）用DNA转化植物或植物细胞的质体，所述DNA包括：（i）赋予复制的核苷酸序列 所述DNA在植物细胞中，（ii）至少一种感兴趣的序列，（iii）侧翼于所述至少一个感兴趣序列侧翼的序列，所述至少一个感兴趣序列用于将所述至少一个感兴趣的序列稳定整合到质体基因组中，以及（iv ）在所述感兴趣的序列侧翼的所述序列之外的选择标记; （b）允许在存在选择压力的情况下将所述至少一个感兴趣序列整合到塑料瓶中; （c）通过释放选择压力允许所述选择标记序列的丢失; 和（d）回收在其塑料上遗传转化并且没有所述选择标记的细胞和/或植物。
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公开(公告)号：US20070300330A1
公开(公告)日：2007-12-27
申请号：US10586998
申请日：2005-01-19
CPC分类号： C12N15/8216
摘要： A process for replicating or for replicating and expressing a sequence of interest in a plant, comprising: (i) an RNA replicon or a precursor thereof, said RNA replicon being derived from a plus-sense single stranded RNA virus and comprising at least one sequence of interest; and (ii) a helper replicon, or a precursor thereof, wherein said helper replicon is (a) incapable of systemic movement in said plant both in the presence and in the absence of said RNA replicon (i) and (b) capable of expressing in a plant one or more proteins necessary for systemic movement of said RNA replicon (i), whereby said RNA replicon (i) is capable of replicating or replicating and expressing said sequence of interest in said plant, but unable to move systemically in said plant in the absence of said one or more proteins expressed by said helper replicon (ii).
摘要翻译： 一种在植物中复制或表达感兴趣的序列的方法，包括：（i）RNA复制子或其前体，所述RNA复制子衍生自正义单链RNA病毒，并且包含至少一个序列 出于兴趣; 辅助复制子或其前体，其中所述辅助复制子是（a）在所述RNA复制子（i）和（b）能够表达的存在和不存在下，在所述植物中不能全身运动 在植物中，所述RNA复制子（i）的系统运动所必需的一种或多种蛋白质，其中所述RNA复制子（i）能够在所述植物中复制或复制和表达所述感兴趣的序列，但不能在所述植物中系统地移动 在不存在由所述辅助复制子（ii）表达的所述一种或多种蛋白质的情况下。
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公开(公告)号：US20060026718A1
公开(公告)日：2006-02-02
申请号：US10535780
申请日：2003-11-20
CPC分类号： C12N15/8216 , C12N15/8217 , C12N15/8237
摘要： A method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly introducing a polypeptide from a cell-free composition into cells containing said heterologous nucleic acid wherein said polypeptide and said heterologous nucleic acid are mutually adapted such that said polypeptide is capable of switching on said cellular process of interest.
摘要翻译： 一种控制遗传修饰植物的方法，包括（a）提供经遗传修饰的植物，其中所述经遗传修饰的植物的细胞含有异源核酸，由此所述遗传修饰的植物在细胞过程中是无活性的 兴趣，（b）通过将多肽从无细胞组合物直接引入到含有所述异源核酸的细胞中，其中所述多肽和所述异源核酸相互适应使得所述多肽能够导通，从而切换所述感兴趣的细胞过程 所述细胞过程是感兴趣的。
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公开(公告)号：US20070124831A1
公开(公告)日：2007-05-31
申请号：US10545665
申请日：2004-01-30
CPC分类号： C12N15/8205
摘要： A process of endowing a plant or plant cells with a trait of interest by expressing an RNA sequence of interest, said process comprising: providing plant cells or cells of said plant with a first vector and a second vector and selecting cells endowed with said trait of interest, wherein said first vector contains a first nucleotide sequence with a first segment coding, in 5′ to 3′ direction, for—a 5′ part of said RNA sequence of interest and—a 5′ part of an intron; and said second vector contains a second nucleotide sequence with a second segment coding, in 5′ to 3′ direction, for—a 3′ part of an intron and—a 3′ part of said RNA sequence of interest.
摘要翻译： 通过表达感兴趣的RNA序列赋予具有感兴趣特征的植物或植物细胞的方法，所述方法包括：向第一载体和第二载体提供所述植物的植物细胞或细胞，并选择赋予所述特征的所述性状的细胞 其中所述第一载体含有第一个核苷酸序列，第一个片段在5'至3'方向上编码所述感兴趣的所述RNA序列的5'部分和内含子的5'部分; 并且所述第二载体含有第二个核苷酸序列，第二个片段在5'至3'方向上编码3'部分内含子和3'部分所述目的RNA序列。
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公开(公告)号：US07193131B2
公开(公告)日：2007-03-20
申请号：US10466221
申请日：2002-01-18
申请人： Christian Eibl , Fong-Chin Huang , Sebastian Klaus , Stefan Mühlbauer , Stefan Herz , Hans-Ulrich Koop
发明人： Christian Eibl , Fong-Chin Huang , Sebastian Klaus , Stefan Mühlbauer , Stefan Herz , Hans-Ulrich Koop
IPC分类号： C12N15/82
CPC分类号： C12N15/8214 , C12N15/8209
摘要： A process for producing multicellular plants, plant organs or plant tissues transformed on their plastome by the following steps is provided: (a) altering or disrupting the function of a gene in a plastid genome for producing a selectable or recognizable phenotype; (b) separating or selecting plants or cells having plastids expressing said phenotype; (c) transforming said plastid genome of said separated or selected plant, plant organ or plant tissue with at least one transformation vector having a restoring sequence capable of restoring said function; and (d) separating or selecting said transformed plant, plant organ or plant tissue having plastids expressing said restored function.
摘要翻译： 提供了通过以下步骤生产多细胞植物，植物器官或其植物组织转化的方法：（a）改变或破坏质体基因组中的基因的功能以产生可选择或可识别的表型; （b）分离或选择具有表达所述表型的质体的植物或细胞; （c）用具有能够恢复所述功能的恢复序列的至少一个转化载体转化所述分离或选择的植物，植物器官或植物组织的所述质体基因组; 和（d）分离或选择具有表达所述恢复功能的质体的所述转化植物，植物器官或植物组织。
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公开(公告)号：US20060075524A1
公开(公告)日：2006-04-06
申请号：US10535763
申请日：2003-11-20
CPC分类号： C12N15/63 , C12N15/67 , C12N15/8216 , C12N15/8217
摘要： A method of controlling a genetically-modified multi-cellular organism or a part thereof, comprising the following steps: (a) providing a multi-cellular organism or a part thereof, whereby cells of said multi-cellular organism or said part contain a heterologous nucleic acid, (b) causing expression of a I protein from said heterologous nucleic acid in at least some of said cells, wherein said protein is capable of (i) leaving a cell and entering other cells of said multi-cellular organism or a part thereof, (ii) causing expression of said protein in cells containing said heterologous nucleic acid, and optionally (iii) controlling a cellular process of interest.
摘要翻译： 一种控制遗传修饰的多细胞生物体或其一部分的方法，包括以下步骤：（a）提供多细胞生物体或其一部分，由此所述多细胞生物体或所述部分的细胞含有异源 核酸，（b）在至少一些所述细胞中引起来自所述异源核酸的I蛋白的表达，其中所述蛋白质能够（i）离开细胞并进入所述多细胞生物体的其它细胞或部分 （ii）引起所述蛋白质在含有所述异源核酸的细胞中的表达，和任选地（iii）控制感兴趣的细胞过程。
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公开(公告)号：US20050221323A1
公开(公告)日：2005-10-06
申请号：US10512920
申请日：2002-04-30
CPC分类号： C12N15/63 , C12N15/67 , C12N15/8203 , C12N15/8216
摘要： This invention provides process of amplification and/or expression of a sequence of interest in a cell by providing for generating within said cell at least one amplicon by trans-splicing between an RNA sequence designed for being capable of trans-splicing and a target RNA, whereby said amplicon is capable of amplifying in said cell and capable of expressing a sequence of interest.
摘要翻译： 本发明提供在细胞中扩增和/或表达感兴趣的序列的方法，其通过在所述细胞内产生至少一个扩增子，通过在设计用于转接的RNA序列与靶RNA之间进行反式剪接， 由此所述扩增子能够在所述细胞中扩增并且能够表达感兴趣的序列。
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公开(公告)号：US20090265814A1
公开(公告)日：2009-10-22
申请号：US10544135
申请日：2004-01-30
CPC分类号： C12N15/8213 , C12N15/8205
摘要： A process of producing transgenic plants or plant cells stably transformed on a chromosome with a DNA sequence of interest and capable of expressing a function of interest from said DNA sequence of interest, said process comprising (a) providing plant cells or plants with at least two different vectors, whereby (i) said at least two different vectors are adapted to recombine with each other by site-specific recombination in said plant cells for producing a non-replicating recombination product containing said DNA sequence of interest, (ii) said at least two different vectors are adapted for integrating said DNA sequence of interest into said chromosome, (iii) said DNA sequence of interest contains sequence portions from at least two of said at least two different vectors, said sequence portions being necessary for expressing said function of interest from said DNA sequence of interest; and (b) selecting plants or plant cells expressing said function of interest.
摘要翻译： 一种生产转基因植物或植物细胞的方法，所述转基因植物或植物细胞在染色体上稳定转化，所述染色体具有感兴趣的DNA序列并且能够从所述感兴趣的DNA序列表达目的功能，所述方法包括（a）向植物细胞或植物提供至少两种 不同的载体，其中（i）所述至少两种不同的载体适于通过所述植物细胞中的位点特异性重组而彼此重组，用于产生含有所述感兴趣的DNA序列的非复制重组产物，（ii）所述至少 两个不同的载体适于将目标DNA序列整合到所述染色体中，（iii）所述感兴趣的DNA序列包含来自所述至少两种不同载体中的至少两种的序列部分，所述序列部分是表达所述感兴趣的功能所必需的 从所述感兴趣的DNA序列; 和（b）选择表达所述目的功能的植物或植物细胞。
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公开(公告)号：US20060272051A1
公开(公告)日：2006-11-30
申请号：US10559430
申请日：2004-06-04
申请人： Stefan Werner , Romy Kandzia , Serik Eliby , Sylvestre Marillonnet , Victor Klimyuk , Yuri Gleba
发明人： Stefan Werner , Romy Kandzia , Serik Eliby , Sylvestre Marillonnet , Victor Klimyuk , Yuri Gleba
CPC分类号： C12N15/8216 , C12N15/8217 , C12N15/8242 , C12N15/8257
摘要： A process of the production of a product of interest in an F1 seed obtained by a hybridization of a first and a second transgenic parental plant, said hybridization generating a genetic endowment in said F1 seed for said production by combining in said F1 seed first and second partial genetic endowments of said first and second transgenic parental plants, followed by isolating said product of interest from said F1 seed or a seedling thereof.
摘要翻译： 通过第一和第二转基因亲本植物的杂交获得的F1种子中感兴趣的产品的生产过程，所述杂交通过在所述F1种子中组合产生用于所述生产的所述F1种子中的遗传禀赋，第一和第二 所述第一和第二转基因亲本植物的部分遗传禀赋，然后从所述F1种子或其幼苗中分离所述目的产物。
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公开(公告)号：US20060174365A1
公开(公告)日：2006-08-03
申请号：US10512879
申请日：2002-04-29
IPC分类号： C12N15/00
CPC分类号： C12N15/8286 , A01K2217/05 , A01K2217/20 , A01K2227/10 , C12N15/8263 , C12N15/8265 , C12N15/8271 , C12N15/8273 , C12N15/8277 , C12N15/85 , C12N15/8509 , C12N2800/30 , C12N2800/40 , C12N2840/10 , C12N2840/445 , Y02A40/162
摘要： A process of producing a transgenic multi-cellular plant or animal organism expressing a trait of interest and having a controlled distribution of said trait to progeny, wherein said process comprises hybridising a first multi-cellular organism or a cell thereof having a first heterologous DNA sequence comprising a first fragment of a nucleotide sequence encoding said trait of interest and a second multi-cellular organism or a cell thereof having a second heterologous DNA sequence comprising a second fragment of the nucleotide sequence encoding said trait of interest, whereby said first and said second heterologous sequences are designed such that said trait of interest arises due to RNA trans-splicing after said hybridation.
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