发明申请
US20090305240A1 METHOD FOR DETECTION OF MICROORGANISM AND KIT FOR DETECTION OF MICROORGANISM
有权
用于检测微生物的方法和用于检测微生物的试剂盒
- 专利标题: METHOD FOR DETECTION OF MICROORGANISM AND KIT FOR DETECTION OF MICROORGANISM
- 专利标题(中): 用于检测微生物的方法和用于检测微生物的试剂盒
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申请号: US11994823申请日: 2006-02-17
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公开(公告)号: US20090305240A1公开(公告)日: 2009-12-10
- 发明人: Shinichi Yoshida , Takashi Soejima
- 申请人: Shinichi Yoshida , Takashi Soejima
- 申请人地址: JP Minato-ku, Tokyo
- 专利权人: Morinaga Milk Industry Co., Ltd.
- 当前专利权人: Morinaga Milk Industry Co., Ltd.
- 当前专利权人地址: JP Minato-ku, Tokyo
- 国际申请: PCT/JP2006/302893 WO 20060217
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
Live cells of a microorganism in a test sample are detected by the following steps: a) the step of treating the test sample with a topoisomerase poison and/or a DNA gyrase poison, b) the step of extracting DNA from the test sample, and amplifying a target region of the extracted DNA by PCR, and c) the step of analyzing an amplification product.
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