公开(公告)号：US10065995B2

公开(公告)日：2018-09-04

申请号：US14007225

申请日：2012-03-26

Applicant: Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira

Inventor： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira

IPC: C07K14/31 ,  C07K1/22 ,  C07K16/00 ,  C07K16/12 ,  C07K17/12 ,  B01D15/38 ,  C07K16/06 ,  C07K17/00

Abstract: An object of the present invention is to provide a technique to create novel engineered protein ligands that, when immobilized through a lysine residue (its side chain ε-amino group) which allows for efficient immobilization to a carrier, show the optimum binding capacity and binding efficiency to a target molecule. The present invention provides an engineered protein having a sequence obtained by replacing all the lysine residues in Protein A, which is the most typical protein ligand, with other amino acids, and adding lysine at a terminal; and an affinity separation matrix in which such an engineered protein is immobilized on a water-insoluble carrier by reductive amination or the like. This affinity separation matrix is characterized by its high binding capacity to a target molecule even when the immobilized amount of the ligand is small.

公开(公告)号：US09108718B2

公开(公告)日：2015-08-18

申请号：US13386737

申请日：2010-10-05

Applicant: Shinichi Yoshida ,  Hideaki Tanaka ,  Yuya Tanaka

Inventor： Shinichi Yoshida ,  Hideaki Tanaka ,  Yuya Tanaka

IPC: B32B3/14 ,  B32B3/24 ,  B32B3/26 ,  B32B7/02 ,  B32B7/08 ,  B64C1/12 ,  B64C1/00 ,  B64C1/06 ,  B64C3/00 ,  B64C3/26 ,  B64C3/18 ,  B64C1/14 ,  B32B7/00 ,  B32B3/08

CPC classification number: B64C3/187 ,  B32B3/08 ,  B32B3/14 ,  B32B3/263 ,  B32B3/266 ,  B32B7/005 ,  B32B7/02 ,  B32B7/08 ,  B64C1/061 ,  B64C1/064 ,  B64C1/068 ,  B64C1/069 ,  B64C1/1446 ,  B64C1/1492 ,  B64C3/182 ,  B64C3/26 ,  B64C2001/0072 ,  Y02T50/43 ,  Y10T428/24322

Abstract: A main wing (1) on which a tensile load is exerted in a longitudinal direction includes a holed structural member that is a composite material made of fiber reinforced plastic which extends in the longitudinal direction and in which access holes (5) are formed and that serves as a center portion (3b); and a front portion (3a) and a rear portion (3c) that are composite materials made of fiber reinforced plastic which extend in the longitudinal direction of the main wing and which are connected to side portions of the center portion. The tensile rigidity of the center portion in the longitudinal direction is set to be lower than the tensile rigidity of the front portion and the rear portion in the longitudinal direction.

Abstract translation: 在纵向上施加拉伸载荷的主翼（1）包括作为由长度方向延伸并且形成有进入孔（5）的由纤维增强塑料制成的复合材料的孔结构构件，并且形成有进入孔（5） 用作中心部分（3b）; 以及作为由所述主翼的长度方向延伸并与所述中心部的侧部连接的由纤维增强塑料制成的复合材料的前部（3a）和后部（3c）。 中心部分在纵向上的拉伸刚度被设定为低于前部和后部在纵向上的拉伸刚度。

公开(公告)号：US08902200B2

公开(公告)日：2014-12-02

申请号：US13589257

申请日：2012-08-20

Applicant: Masayuki Miyamoto ,  Shinichi Yoshida ,  Akira Nishioka ,  Manabu Yumoto ,  Kengo Takahama

Inventor： Masayuki Miyamoto ,  Shinichi Yoshida ,  Akira Nishioka ,  Manabu Yumoto ,  Kengo Takahama

IPC: G06F3/042

CPC classification number: G06F3/044 ,  G06F3/0416 ,  G06F3/0418 ,  G06F2203/04112

Abstract: A touch panel system includes a touch panel having sense lines, and drive lines which intersect the sense lines and form capacitance with the sense lines, a touch panel controller for processing signals of the sense lines, and a drive line driving circuit for driving the drive lines in parallel. The touch panel controller includes a subtracting section for finding differences in signals between the sense lines adjacent to each other, a decoding section for calculating a distribution of differences between the capacitances by calculating an inner product of code sequences for driving the drive lines in parallel and difference output sequences which correspond to the code sequences, a touch detecting section for obtaining touch information based on the distribution of differences between the capacitances calculated by the decoding section, and a region setting section for setting an effective region in the touch panel based on the touch information.

Abstract translation: 触摸面板系统包括具有感测线的触摸面板和与感测线相交并与感测线形成电容的驱动线，用于处理感测线的信号的触摸面板控制器和用于驱动驱动器的驱动线驱动电路 行并行。 触摸面板控制器包括用于求出彼此相邻的感测线之间的信号差异的减法部分，用于通过计算并行驱动驱动线的代码序列的内积来计算电容之间的差分布的解码部分，以及 对应于代码序列的差分输出序列，用于基于由解码部分计算出的电容之间的差异的分布获得触摸信息的触摸检测部分和用于基于触摸面板设置触摸面板中的有效区域的区域设置部分 触摸信息。

公开(公告)号：US20140132541A1

公开(公告)日：2014-05-15

申请号：US14127932

申请日：2012-06-04

Applicant: Masayuki Miyamoto ,  Kunihiko Iizuka ,  Manabu Yumoto ,  Shinichi Yoshida ,  Kengo Takahama

Inventor： Masayuki Miyamoto ,  Kunihiko Iizuka ,  Manabu Yumoto ,  Shinichi Yoshida ,  Kengo Takahama

IPC: G06F3/041

CPC classification number: G06F3/041 ,  G06F3/0418 ,  G06F3/044

Abstract: A touch panel system (71a) includes a capacitance value distribution detection circuit (72). The capacitance value distribution detection circuit (72) switches a connection state between a first connection state and a second connection state, which first connection state makes first signal lines (HL1 to HLM) serve as drive lines (DL1 to DLM) and second signal lines (VL1 to VLM) serve as sense lines (SL1 to SLM), and which second connection state makes the second signal lines (VL1 to VLM) serve as the drive lines (DL1 to DLM) and the first signal lines (HL1 to HLM) serve as the sense lines (SL1 to SLM).

Abstract translation: 触摸面板系统（71a）包括电容值分配检测电路（72）。 电容值分配检测电路（72）在第一连接状态和第二连接状态之间切换连接状态，第一连接状态使第一信号线（HL1〜HLM）成为驱动线（DL1〜DLM）和第二信号线 （VL1〜VLM）作为感测线（SL1〜SLM），第二连接状态使第二信号线（VL1〜VLM）成为驱动线（DL1〜DLM），第一信号线（HL1〜HLM） 用作感测线（SL1至SLM）。

公开(公告)号：US20140018524A1

公开(公告)日：2014-01-16

申请号：US14007000

申请日：2012-03-23

Applicant: Dia Murata ,  Shinichi Yoshida

Inventor： Dia Murata ,  Shinichi Yoshida

IPC: C07K17/00

CPC classification number: C07K17/00 ,  B01D15/3809 ,  B01J20/289 ,  B01J20/3202 ,  B01J20/3219 ,  B01J20/3246 ,  C07K1/22

Abstract: The low-molecular-weight compound represented by the general formula (1): ArX-(Linker)-ArYHB (1), wherein ArX is a structure containing an optionally substituted aromatic six-membered ring, ArYHB is a structure containing an optionally substituted aromatic six-membered ring having a proton donor, the atom group “Linker” has not less than 4 and not more than 30 atoms and binds ArX with ArYHB, is used for binding a proteinaceous substance.

Abstract translation: 由通式（1）表示的低分子量化合物：ArX-（连接子）-ArYHB（1），其中ArX是含有任意取代的芳香族六元环的结构，ArYHB是含有任意取代的 具有质子给体的芳族六元环，原子团“连接子”具有不少于4个且不多于30个的原子并与ArYHB结合ArX，用于结合蛋白质物质。

公开(公告)号：US20130243992A1

公开(公告)日：2013-09-19

申请号：US13881620

申请日：2012-02-03

Applicant: Yuya Tanaka ,  Shinichi Yoshida ,  Hideaki Tanaka ,  Hideyuki Suzuki ,  Toshio Abe ,  Masahiro Kashiwagi

Inventor： Yuya Tanaka ,  Shinichi Yoshida ,  Hideaki Tanaka ,  Hideyuki Suzuki ,  Toshio Abe ,  Masahiro Kashiwagi

IPC: B64C1/14

CPC classification number: B64C1/14 ,  B29C70/887 ,  B32B3/266 ,  B32B5/142 ,  B32B5/26 ,  B32B27/08 ,  B32B2260/023 ,  B32B2260/046 ,  B32B2262/0269 ,  B32B2262/101 ,  B32B2262/106 ,  B32B2307/54 ,  B32B2605/18 ,  B64C3/182 ,  B64C3/20 ,  B64C3/34 ,  B64C2001/0072 ,  Y02T50/43 ,  Y10T428/192 ,  Y10T428/24273

Abstract: A composite material structure that can be made lighter in weight is provided, with the stress concentration at peripheral edge regions around holes being taken into consideration. A wing (1) that is a composite material structure is of a composite material that extends in one direction, has access holes (5) formed therein, and is made of fiber reinforced plastic. A lower surface outer plate (3) of the wing (1) is subjected to a tensile load in the longitudinal direction. The longitudinal tensile stiffness of peripheral edge regions (3a) around the access holes (5) is lower than the longitudinal tensile stiffness of other regions (3b) surrounding the peripheral edge regions (3a).

Abstract translation: 提供可以使重量更轻的复合材料结构，考虑到孔附近的周边边缘区域处的应力集中。 作为复合材料结构的翼（1）是沿一个方向延伸的复合材料，其中形成有入口孔（5），并且由纤维增强塑料制成。 机翼（1）的下表面外板（3）沿长度方向承受拉伸载荷。 周围边缘区域（3a）围绕进入孔（5）的纵向拉伸刚度低于围绕周边边缘区域（3a）的其它区域（3b）的纵向拉伸刚度。

公开(公告)号：US20120277121A1

公开(公告)日：2012-11-01

申请号：US13523738

申请日：2012-06-14

Applicant: Shinichi Yoshida ,  Takashi Soejima

Inventor： Shinichi Yoshida ,  Takashi Soejima

IPC: C12Q1/68 ,  C40B40/06

CPC classification number: C12Q1/68 ,  C12Q1/689 ,  C12Q2523/101

Abstract: A kit for use in a method for detecting live cells, injured cells and dead cells of a microorganism in a test sample by a nucleic acid amplification method is disclosed. The kit includes a cross-linker capable of cross-linking DNA by irradiation with light having a wavelength of 350 nm to 700 nm, medium, and a primer (s) for amplifying a DNA target region in the microorganism by a nucleic acid amplification method. The nucleic acid amplification method may be a PCR, LAMP, SDA, LCR or DNA microarray method. A cross-linker may be included in the kit such as ethidium monoazide, ethidium diazide, psoralen, 4,5′,8-trimethyl psoralen, or 8-methoxy psoralen.

Abstract translation: 公开了一种用于通过核酸扩增方法检测测试样品中微生物的活细胞，受损细胞和死细胞的方法的试剂盒。 试剂盒包括能够通过用波长为350nm至700nm的光照射DNA和通过核酸扩增方法扩增微生物中的DNA靶标区域的引物的交联剂 。 核酸扩增方法可以是PCR，LAMP，SDA，LCR或DNA微阵列法。 试剂盒中可以包括交联剂，例如单乙叠胺，二氮嗪，补骨脂素，4,5'，8-三甲基补骨脂素或8-甲氧基补骨脂素。

公开(公告)号：US08241866B2

公开(公告)日：2012-08-14

申请号：US12832740

申请日：2010-07-08

Applicant: Shinichi Yoshida ,  Takashi Soejima

Inventor： Shinichi Yoshida ,  Takashi Soejima

IPC: C12Q1/02 ,  C12Q1/04 ,  C12Q1/18

CPC classification number: C12Q1/04 ,  C12Q1/34 ,  C12Q1/533 ,  C12Q2304/10

Abstract: A kit is disclosed for preparing a measurement sample for detecting live cells, injured cells, VNC cells and dead microorganism cells in a test sample by the following steps: a) the step of treating the test sample with an enzyme having an activity of decomposing cells other than those of the microorganism, colloidal particles of proteins or lipids existing in the test sample, b) the step of treating the test sample with a topoisomerase poison and/or a DNA gyrase poison. c) the step of treating the test sample treated in the steps a) and b) with a nuclear stain agent, and d) the step of detecting the microorganism in the test sample treated with the nuclear stain agent by flow cytometry.

Abstract translation: 公开了用于通过以下步骤制备用于检测测试样品中的活细胞，受损细胞，VNC细胞和死亡微生物细胞的测量样品的试剂盒：a）用具有分解细胞活性的酶处理测试样品的步骤 除了微生物之外，存在于测试样品中的蛋白质或脂质的胶体颗粒，b）用拓扑异构酶毒物和/或DNA回旋酶毒物处理测试样品的步骤，c）处理测试样品的步骤 在步骤a）和b）中使用核染色剂，以及d）通过流式细胞术检测用核染色剂处理的测试样品中的微生物的步骤。

公开(公告)号：US20120121854A1

公开(公告)日：2012-05-17

申请号：US13386737

申请日：2010-10-05

Applicant: Shinichi Yoshida ,  Hideaki Tanaka ,  Yuya Tanaka

Inventor： Shinichi Yoshida ,  Hideaki Tanaka ,  Yuya Tanaka

IPC: B32B3/10

CPC classification number: B64C3/187 ,  B32B3/08 ,  B32B3/14 ,  B32B3/263 ,  B32B3/266 ,  B32B7/005 ,  B32B7/02 ,  B32B7/08 ,  B64C1/061 ,  B64C1/064 ,  B64C1/068 ,  B64C1/069 ,  B64C1/1446 ,  B64C1/1492 ,  B64C3/182 ,  B64C3/26 ,  B64C2001/0072 ,  Y02T50/43 ,  Y10T428/24322

Abstract: An object is to provide a main wing whose weight can be reduced, taking into consideration the stress concentration at circumferential edges of access holes. A main wing (1) on which a tensile load is exerted in a longitudinal direction includes a holed structural member that is a composite material made of fiber reinforced plastic which extends in the longitudinal direction and in which access holes (5) are formed and that serves as a center portion (3b); and a front portion (3a) and a rear portion (3c) that are composite materials made of fiber reinforced plastic which extend in the longitudinal direction of the main wing 1 and which are connected to side portions of the center portion (3b). The tensile rigidity of the center portion (3b) in the longitudinal direction is set to be lower than the tensile rigidity of the front portion (3a) and the rear portion (3c) in the longitudinal direction.

Abstract translation: 目的是提供一种能够减小重量的主翼，同时考虑到进入孔的圆周边缘处的应力集中。 在纵向上施加拉伸载荷的主翼（1）包括作为由长度方向延伸并且形成有进入孔（5）的由纤维增强塑料制成的复合材料的孔结构构件，并且形成有进入孔（5） 用作中心部分（3b）; 以及作为由主翼1的长度方向延伸并与中央部3b的侧部连接的纤维增强塑料制的复合材料的前部3a和后部3c。 中心部分（3b）在纵向上的拉伸刚度被设定为低于前部（3a）和后部（3c）在纵向上的拉伸刚度。

公开(公告)号：US08143018B2

公开(公告)日：2012-03-27

申请号：US12832782

申请日：2010-07-08

Applicant: Shinichi Yoshida ,  Takashi Soejima

Inventor： Shinichi Yoshida ,  Takashi Soejima

IPC: C12Q1/02 ,  C12Q1/04 ,  C12Q1/18

CPC classification number: C12Q1/04 ,  C12Q1/34 ,  C12Q1/533 ,  C12Q2304/10

Abstract: According to the following steps, live cells, injured cells, VNC cells and dead cells of a microorganism in a test sample are detected by flow cytometry: a) the step of treating the test sample with an enzyme having an activity of decomposing cells other than those of the microorganism, colloidal particles of proteins or lipids existing in the test sample, b) the step of treating the test sample with a topoisomerase poison and/or a DNA gyrase poison. c) the step of treating the test sample treated in the steps a) and b) with a nuclear stain agent, and d) the step of detecting the microorganism in the test sample treated with the nuclear stain agent by flow cytometry.

Abstract translation: 根据以下步骤，通过流式细胞术检测试验样品中微生物的活细胞，受损细胞，VNC细胞和死细胞：a）用具有分解细胞以外的细胞活性的酶处理测试样品的步骤 微生物的那些，存在于测试样品中的蛋白质或脂质的胶体颗粒，b）用拓扑异构酶毒物和/或DNA回旋酶毒物处理测试样品的步骤，c）处理在测试样品中处理的测试样品的步骤 步骤a）和b），以及d）通过流式细胞术检测用核染色剂处理的测试样品中的微生物的步骤。