Invention Application
US20090305252A1 Methods and species-specific primers for detection and quantification of Streptococcus mutans and Streptococcus sanguinis in mixed bacterial samples
审中-公开
在混合细菌样品中检测和定量变异链球菌和血链球菌的方法和物种特异性引物
- Patent Title: Methods and species-specific primers for detection and quantification of Streptococcus mutans and Streptococcus sanguinis in mixed bacterial samples
- Patent Title (中): 在混合细菌样品中检测和定量变异链球菌和血链球菌的方法和物种特异性引物
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Application No.: US12156955Application Date: 2008-06-04
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Publication No.: US20090305252A1Publication Date: 2009-12-10
- Inventor: Yihong Li , Page W. Caufield , Deepak Saxena , Zhou Chen
- Applicant: Yihong Li , Page W. Caufield , Deepak Saxena , Zhou Chen
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H21/00
Abstract:
Dental caries is a polymicrobial infectious disease. Of the hundreds of bacteria present in the biofilms coating teeth, the Streptococcus mutans (S. mutans) remain strongly linked to caries and dental disease. Streptococcus sanguinis (S. sanguinis) may serve a protective or antagonistic role against the cariogenic bacterium S. mutans. In the present invention, exemplary sets of species-specific PCR primers are provided for the identification and quantification of S. mutans and of S. sanguinis in clinical samples, including the simultaneous and sensitive analysis of both bacterial species. Assays, kits and methods for determining the presence and amount of S. mutans and/or S. sanguinis are provided. Oligonucleotide probes and primers for use in the assays, kits and methods are described. Assays and methods for determining and evaluating an individual's oral bacteria, risk for caries, and effects of prevention and treatment modalities, are provided.
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