发明申请
- 专利标题： METHOD OF CHARACTERIZING ENDOGENOUS POLYNUCLEOTIDE-POLYPEPTIDE INTERACTIONS
- 专利标题（中）： 表征内源多核苷酸多肽交互的方法
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申请号： US12348630申请日： 2009-01-05
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公开(公告)号： US20090305272A1公开(公告)日： 2009-12-10
- 发明人: Zhengne Wang , Xiaodong Zhang
- 申请人： Zhengne Wang , Xiaodong Zhang
- 主分类号： C12Q1/68
- IPC分类号： C12Q1/68 ; G01N33/53 ; C12N15/63
摘要：
A method for characterizing an endogenous polypeptide includes introducing epitope tag-encoding polynucleotide into an endogenous locus of a somatic cell by homogenous recombination mediated knock-in so that an epitope tagged endogenous polypeptide is expressed by the cell, and characterizing the epitope tagged endogenous polypeptide using an immunoassay.
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