METHOD OF CHARACTERIZING ENDOGENOUS POLYNUCLEOTIDE-POLYPEPTIDE INTERACTIONS
    1.
    发明申请
    METHOD OF CHARACTERIZING ENDOGENOUS POLYNUCLEOTIDE-POLYPEPTIDE INTERACTIONS 审中-公开
    表征内源多核苷酸多肽交互的方法

    公开(公告)号:US20090305272A1

    公开(公告)日:2009-12-10

    申请号:US12348630

    申请日:2009-01-05

    IPC分类号: C12Q1/68 G01N33/53 C12N15/63

    CPC分类号: G01N33/5308 G01N33/536

    摘要: A method for characterizing an endogenous polypeptide includes introducing epitope tag-encoding polynucleotide into an endogenous locus of a somatic cell by homogenous recombination mediated knock-in so that an epitope tagged endogenous polypeptide is expressed by the cell, and characterizing the epitope tagged endogenous polypeptide using an immunoassay.

    摘要翻译: 用于表征内源性多肽的方法包括通过均质重组介导的敲入将表达标签编码多核苷酸引入体细胞的内源基因座,使得表位标记的内源多肽由细胞表达,并使用 免疫测定