发明申请
US20090305359A1 METHOD FOR PRODUCING CIRCULAR DUPLEX POLYNUCLEOTIDES FROM LINEAR DUPLEX POLYNUCLEOTIDES AND APPLICATIONS THEREOF
审中-公开
用于从线性双重多晶硅生产圆形双重多晶硅的方法及其应用
- 专利标题： METHOD FOR PRODUCING CIRCULAR DUPLEX POLYNUCLEOTIDES FROM LINEAR DUPLEX POLYNUCLEOTIDES AND APPLICATIONS THEREOF
- 专利标题（中）： 用于从线性双重多晶硅生产圆形双重多晶硅的方法及其应用
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申请号： US12466236申请日： 2009-05-14
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公开(公告)号： US20090305359A1公开(公告)日： 2009-12-10
- 发明人: G. Wesley Hatfield , Liza S.Z. Larsen , Richard H. Lathrop
- 申请人： G. Wesley Hatfield , Liza S.Z. Larsen , Richard H. Lathrop
- 主分类号： C12P19/34
- IPC分类号： C12P19/34 ; C12N15/87 ; C07H21/00
摘要：
Methods are provided for making a circular duplex polynucleotide. Such methods can include providing a mixture of sequence specific linear duplex polynucleotides and denaturing and reannealing the polynucleotide mixture under conditions such that some of the polynucleotides form circular duplexes that comprise the desired polynucleotide.
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