发明申请
- 专利标题: Compositions and Methods to Monitor RNA Delivery to Cells
- 专利标题(中): 组织和方法监测细胞的RNA传递
-
申请号: US12096344申请日: 2006-12-08
-
公开(公告)号: US20090317802A1公开(公告)日: 2009-12-24
- 发明人: Sangeeta N. Bhatia , Austin M. Derfus , Alice A. Chen
- 申请人: Sangeeta N. Bhatia , Austin M. Derfus , Alice A. Chen
- 国际申请: PCT/US06/46852 WO 20061208
- 主分类号: C12Q1/68
- IPC分类号: C12Q1/68
摘要:
Methods and compositions for tracking or monitoring uptake of siRNA by mammalian cells are provided. The methods and compositions may be used to monitoring the silencing activity of the internalized siRNA. The compositions contain an siRNA, an optically or magnetically detectable nanoparticle such as a quantum dot and, optionally, a transfection reagent. Cells are contacted with an siRNA and an optically or magnetically detectable nanoparticle, optionally in the presence of a transfection reagent. Detection of internalized nanoparticles is indicative of siRNA uptake. The invention allows analysis, identification, processing, etc., of cells that have efficiently taken up siRNA. In one embodiment, cells are sorted into at least two populations based on the amount of siRNA taken up.