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    CONTROL OF MATERIALS AND POROUS MAGNETIC PARTICLES
    2.
    发明申请
    CONTROL OF MATERIALS AND POROUS MAGNETIC PARTICLES 有权
    材料和多孔磁性颗粒的控制

    公开(公告)号：US20090179171A1

    公开(公告)日：2009-07-16

    申请号：US12300369

    申请日：2007-05-16

    申请人： Michael J. Sailor ,  Ji-Ho Park ,  Austin M. Derfus ,  Ester Segal ,  Kenneth S. Vecchio ,  Sangeeta N. Bhatia

    发明人： Michael J. Sailor ,  Ji-Ho Park ,  Austin M. Derfus ,  Ester Segal ,  Kenneth S. Vecchio ,  Sangeeta N. Bhatia

    IPC分类号： H01F1/00 ,  H05B6/02

    CPC分类号： H05B6/02 ,  B82Y25/00 ,  H01F1/0063 ,  Y10T428/29 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/32

    摘要： The present invention uses externally applied electromagnetic stimulus to control and heat porous magnetic particles and material associated with the particles. The particles contain magnetic material, such as superparamagnetic iron oxide and are associated with a material. Application of a DC magnetic field allows them to be moved with their associated material, and application of an AC RF electromagnetic field allows them to be heated with their associated material. The material can be associated with the particles by being contained in the pores of the particles, or in other cases the particles can adhere to the associated material, which can be an aqueous droplet. The present invention also provides a multi-layer porous magnetic particle. The particle includes a host layer having pores sized to accept magnetic nanoparticles. Magnetic nanoparticles are infused within pores of the host layer An encoding layer includes pores that define a spectral code. The pores in the encoding layer are sized to substantially exclude the magnetic nanoparticles. The encoding layer can also be a multi-layer, exhibiting, for example, a complex spectral code.

    摘要翻译： 本发明使用外部施加的电磁刺激来控制和加热与颗粒相关联的多孔磁性颗粒和材料。 颗粒含有磁性材料，例如超顺磁性氧化铁，并与材料相关联。 DC磁场的应用允许它们与其相关材料一起移动，并且AC RF电磁场的应用允许它们与其相关材料一起被加热。 该材料可以通过包含在颗粒的孔中与颗粒相关联，或者在其它情况下，颗粒可以附着到可以是水性液滴的相关材料上。 本发明还提供一种多层多孔磁性颗粒。 颗粒包括具有尺寸以接受磁性纳米颗粒的孔的主体层。 磁性纳米颗粒注入到主体层的孔内编码层包括限定光谱代码的孔。 编码层中的孔的大小被确定为基本排除磁性纳米颗粒。 编码层也可以是多层，表现出例如复谱谱。

    Multi-well micropatterning by ablation
    3.
    发明申请
    Multi-well micropatterning by ablation 审中-公开
    消融多孔微图案

    公开(公告)号：US20080220516A1

    公开(公告)日：2008-09-11

    申请号：US11974341

    申请日：2007-10-12

    申请人： David T. Eddington ,  Sangeeta N. Bhatia

    发明人： David T. Eddington ,  Sangeeta N. Bhatia

    IPC分类号： C12M1/22 ,  B44C1/22

    CPC分类号： G01N33/5067 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00587 ,  B01J2219/0898 ,  B01L3/5025 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0636 ,  C12M23/12 ,  C12M23/20 ,  C12M25/04 ,  C12N5/0671 ,  C12N2502/14 ,  C12N2535/10 ,  G01N33/5008 ,  G01N33/54353 ,  G01N33/5436

    摘要： The present invention is drawn to the generation of micropatterns of biomolecules and cells on standard laboratory materials through selective ablation of a physisorbed biomolecule with oxygen plasma. In certain embodiments, oxygen plasma is able to ablate selectively physisorbed layers of biomolecules (e.g., type-I collagen, fibronectin, laminin, and Matrigel) along complex non-linear paths which are difficult or impossible to pattern using alternative methods. In addition, certain embodiments of the present invention relate to the micropatterning of multiple cell types on curved surfaces, multiwell plates, and flat bottom flasks. The invention also features kits for use with the subject methods.

    摘要翻译： 本发明涉及通过用氧等离子体选择性消融物理吸附的生物分子来生成标准实验室材料上的生物分子和细胞的微图案。 在某些实施方案中，氧等离子体能够沿着复杂的非线性路径选择性地吸收生物分子（例如，I型胶原，纤连蛋白，层粘连蛋白和Matrigel）的物理吸附层，这些复杂的非线性路径难以或不可能使用替代方法进行模式化。 此外，本发明的某些实施例涉及在曲面，多孔板和平底烧瓶上的多种细胞类型的微图案化。 本发明还具有用于本发明方法的试剂盒。

    Control of materials and porous magnetic particles
    6.
    发明授权
    Control of materials and porous magnetic particles 有权
    材料和多孔磁性颗粒的控制

    公开(公告)号：US08377147B2

    公开(公告)日：2013-02-19

    申请号：US12300369

    申请日：2007-05-16

    申请人： Michael J. Sailor ,  Ji-Ho Park ,  Austin Derfus ,  Ester Segal ,  Kenneth S. Vecchio ,  Sangeeta N. Bhatia

    发明人： Michael J. Sailor ,  Ji-Ho Park ,  Austin Derfus ,  Ester Segal ,  Kenneth S. Vecchio ,  Sangeeta N. Bhatia

    IPC分类号： B32B15/00 ,  B44C1/22

    CPC分类号： H05B6/02 ,  B82Y25/00 ,  H01F1/0063 ,  Y10T428/29 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/32

    摘要： The present invention uses externally applied electromagnetic stimulus to control and heat porous magnetic particles and material associated with the particles. The particles contain magnetic material, such as superparamagnetic iron oxide and are infused with a material. Application of a DC magnetic field allows them to be moved with their infused material, and application of an AC RF electromagnetic field allows them to be heated with their infused material. The material can be infused into pores of the particles and the particles can also adhere to an aqueous droplet. The present invention also provides a multi-layer porous magnetic particle. The particle includes a host layer having pores sized to accept magnetic nanoparticles. Magnetic nanoparticles are infused within pores of the host layer. An encoding layer includes pores that define a spectral code. The pores in the encoding layer are sized to substantially exclude the magnetic nanoparticles. The encoding layer can also be a multi-layer, exhibiting, for example, a complex spectral code.

    摘要翻译： 本发明使用外部施加的电磁刺激来控制和加热与颗粒相关联的多孔磁性颗粒和材料。 颗粒包含磁性材料，例如超顺磁性氧化铁，并且注入材料。 直流磁场的应用使得它们可以通过其输入的材料移动，并且使用AC RF电磁场允许它们与其输入的材料一起被加热。 该材料可以注入到颗粒的孔中，并且颗粒也可以粘附到水滴上。 本发明还提供一种多层多孔磁性颗粒。 颗粒包括具有尺寸以接受磁性纳米颗粒的孔的主体层。 磁性纳米颗粒注入主体层的孔内。 编码层包括限定光谱代码的孔。 编码层中的孔的大小被确定为基本排除磁性纳米颗粒。 编码层也可以是多层，表现出例如复谱谱。

    MICROMECHANICAL DEVICES FOR CONTROL OF CELL-CELL INTERACTION, AND METHODS OF USE THEREOF
    8.
    发明申请
    MICROMECHANICAL DEVICES FOR CONTROL OF CELL-CELL INTERACTION, AND METHODS OF USE THEREOF 有权
    用于控制细胞间相互作用的微生物装置及其使用方法

    公开(公告)号：US20100167330A1

    公开(公告)日：2010-07-01

    申请号：US12449994

    申请日：2008-03-10

    申请人： Sangeeta N. Bhatia ,  Elliot Hui

    发明人： Sangeeta N. Bhatia ,  Elliot Hui

    IPC分类号： C12Q1/02 ,  C12N5/071 ,  C12N5/0735 ,  C12N5/073 ,  C12N5/02 ,  C12N5/074

    CPC分类号： C12M35/08 ,  C12M23/04 ,  C12M23/20

    摘要： The development and function of living tissues depends largely on interactions between cells that can vary in both time and space; however, temporal control of cell-cell interaction is experimentally challenging. By employing a micromachined silicon substrate with moving parts, herein is disclosed the dynamic regulation of cell-cell interactions via direct manipulation of adherent cells with micron-scale precision. The inventive devices and methods allow mechanical control of both tissue composition and spatial organization. The inventive device and methods enable the investigation of dynamic cell-cell interaction in a multitude of applications, such as intercellular communication, spanning embryogenesis, homeostasis, and pathogenic processes.

    摘要翻译： 活组织的发育和功能在很大程度上取决于时间和空间可能变化的细胞之间的相互作用; 然而，细胞 - 细胞相互作用的时间控制在实验上是具有挑战性的。 通过采用具有移动部件的微加工硅衬底，本文公开了通过以微米级精度直接操纵贴壁细胞的细胞 - 细胞相互作用的动态调节。 本发明的装置和方法允许组织组成和空间组织的机械控制。 本发明的装置和方法能够在多种应用中进行动态细胞 - 细胞相互作用的研究，例如细胞间通讯，跨越胚胎发生，体内平衡和致病过程。

    Compositions and Methods to Monitor RNA Delivery to Cells
    9.
    发明申请
    Compositions and Methods to Monitor RNA Delivery to Cells 审中-公开
    组织和方法监测细胞的RNA传递

    公开(公告)号：US20090317802A1

    公开(公告)日：2009-12-24

    申请号：US12096344

    申请日：2006-12-08

    申请人： Sangeeta N. Bhatia ,  Austin M. Derfus ,  Alice A. Chen

    发明人： Sangeeta N. Bhatia ,  Austin M. Derfus ,  Alice A. Chen

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/111 ,  A61K49/0002 ,  A61K49/0043 ,  A61K49/0054 ,  A61K49/0056 ,  A61K49/0067 ,  B82Y5/00 ,  B82Y15/00 ,  C12N15/87 ,  C12N2310/14 ,  C12N2310/351 ,  C12N2310/3517 ,  C12N2320/10 ,  G01N33/588

    摘要： Methods and compositions for tracking or monitoring uptake of siRNA by mammalian cells are provided. The methods and compositions may be used to monitoring the silencing activity of the internalized siRNA. The compositions contain an siRNA, an optically or magnetically detectable nanoparticle such as a quantum dot and, optionally, a transfection reagent. Cells are contacted with an siRNA and an optically or magnetically detectable nanoparticle, optionally in the presence of a transfection reagent. Detection of internalized nanoparticles is indicative of siRNA uptake. The invention allows analysis, identification, processing, etc., of cells that have efficiently taken up siRNA. In one embodiment, cells are sorted into at least two populations based on the amount of siRNA taken up.

    摘要翻译： 提供了用于跟踪或监测哺乳动物细胞摄取siRNA的方法和组合物。 方法和组合物可用于监测内化siRNA的沉默活性。 组合物含有siRNA，光学或磁性可检测的纳米颗粒，例如量子点，以及任选的转染试剂。 任选地在转染试剂存在下，将细胞与siRNA和光学或磁性可检测的纳米颗粒接触。 内在纳米粒子的检测表明siRNA摄取。 本发明允许有效摄取siRNA的细胞的分析，鉴定，加工等。 在一个实施方案中，基于所摄取的siRNA的量将细胞分选到至少两个群体中。