A system and method for sound source separation. The system and method use a beamforming technique. The sound source separation system includes a windowing processor; a DFT transformer; a transfer function estimator; and a noise estimator. The system also includes a voice signal extractor that cancels individual voice signals, except an individual voice signal that is desired to be extracted among individual voice signals, from the integrated voice signals. The system further includes a voice signal detector that cancels a noise part provided through the noise estimator from a transfer function of an individual voice signal which is desired to be detected and extracts a noise-canceled individual voice signal. Even when two or more sound sources are simultaneously input, the sound sources can be separated from each other and separately stored and managed, or an initial sound source can be stored and managed.