发明申请
US20100029791A1 METHOD FOR PRODUCING VESICLE, VESICLE OBTAINED BY THE PRODUCTION METHOD, AND METHOD FOR PRODUCING FROZEN PARTICLE USED IN PRODUCTION OF VESICLE
失效
用于生产由生产方法获得的VESICLE,VESICLE的方法和用于生产用于生产VESICLE的冷冻颗粒的方法
- 专利标题: METHOD FOR PRODUCING VESICLE, VESICLE OBTAINED BY THE PRODUCTION METHOD, AND METHOD FOR PRODUCING FROZEN PARTICLE USED IN PRODUCTION OF VESICLE
- 专利标题(中): 用于生产由生产方法获得的VESICLE,VESICLE的方法和用于生产用于生产VESICLE的冷冻颗粒的方法
-
申请号: US12449882申请日: 2008-03-03
-
公开(公告)号: US20100029791A1公开(公告)日: 2010-02-04
- 发明人: Sosaku Ichikawa , Takashi Kuroiwa
- 申请人: Sosaku Ichikawa , Takashi Kuroiwa
- 申请人地址: JP Tsukuba-shi, Ibaraki
- 专利权人: University of Tsukuba
- 当前专利权人: University of Tsukuba
- 当前专利权人地址: JP Tsukuba-shi, Ibaraki
- 优先权: JP2007-053089 20070302
- 国际申请: PCT/JP2008/053740 WO 20080303
- 主分类号: B01J13/08
- IPC分类号: B01J13/08 ; B01F3/08
摘要:
A W/O emulsion is produced from an aqueous solution containing a substance to be entrapped in a vesicle in a dissolved or suspended state and an oil phase containing an emulsifier; subsequently, the W/O emulsion is cooled to a temperature at which the aqueous solution of the W/O emulsion becomes a frozen particle and the oil phase maintains a liquid state, and the oil phase is removed; thereafter, an oil phase containing a vesicle constituent lipid is added to the frozen particle, and the obtained mixture is then stirred, so as to substitute the emulsifier on the surface of the frozen particle with the vesicle constituent lipid; and thereafter, an external water phase is added to the frozen particle coated with a lipid membrane, so as to hydrate the lipid membrane by the external water phase. This process achieves a high entrapment yield of a desired substance while controlling desired physical properties such as particle diameter.