A sampling system includes a steam source, a steam valve connected the steam source, a sampling valve connected to the fluid sample source, an isolation valve, a processing module, a drain valve, a drain, and a controller. The controller to passes cleaning fluid from the processing module through the isolation valve to the drain, passes steam through the steam valve, sampling valve, and isolation valve to the drain for a duration sufficient to sterilize the sampling valve, the isolation valve, and a fluid path therebetween, and passes fluid samples from the fluid sample source through the sampling valve and isolation valve to the processing module. The system and a method described delivers safer, more consistent sampling, while reducing the risk of contamination during extraction of a sample from a vessel and minimizing waste of the sample.