An ESD protection device includes a substrate with a doped well of a first conductive type, a first and a second doping region of the first conductive type and a third and a fourth doping region of a second conductive type respectively disposed in the doped well, a first gate disposed on the substrate and between the first and the second doping region, and a second gate disposed on the substrate and between the second and the third doping region to determine the distance between the second and the third doping region in order to precisely adjust the breakdown voltage of the ESD protection device of the present invention.