A wearable, protective barrier comprising a garment having removably attached covers to protect hands and/or instruments placed therein. The covers can be removably affixed to various locations on the garment that would allow them to be detached from the garment before or after insertion of a hand. Various tabs located on the covers aid in inserting and adjusting the covers on the hand and/or instrument. In a specific embodiment, the covers and/or garment comprise one or more heat sensitive materials, such that some portion of a cover can be temporarily heat-sealed to the garment for later removal. In a further embodiment, the garment and covers are sterile and disposable.