发明申请
US20100092966A1 METHOD FOR DETECTING PYROPHOSPHATE BY MEANS OF BIOLUMINESCENCE DETECTION
有权
通过生物荧光检测方法检测磷酸酯的方法
- 专利标题: METHOD FOR DETECTING PYROPHOSPHATE BY MEANS OF BIOLUMINESCENCE DETECTION
- 专利标题(中): 通过生物荧光检测方法检测磷酸酯的方法
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申请号: US12515218申请日: 2007-11-07
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公开(公告)号: US20100092966A1公开(公告)日: 2010-04-15
- 发明人: Nils Burkhardt , Stefan Heitmeier
- 申请人: Nils Burkhardt , Stefan Heitmeier
- 优先权: DE10-2006-054-562.1 20061120
- 国际申请: PCT/EP07/09625 WO 20071107
- 主分类号: C12Q1/66
- IPC分类号: C12Q1/66 ; C40B30/08 ; C12Q1/68 ; C12M1/34
摘要:
The invention relates to methods for detecting pyrophosphate by means of bioluminescence detection. In addition, methods for measuring chemical, especially enzyme-catalyzed, reactions in which pyrophosphate is formed or consumed are described. Such reactions are catalyzed for example by guanylyl cyclases, adenylyl cyclases, DNA polymerases or RNA polymerases.The novel methods are distinguished by high sensitivity and low susceptibility to interference, can easily be automated and miniaturized and are additionally suitable for carrying out continuous measurements. The methods can be employed particularly advantageously in the area of medical diagnosis and biomedical research, including pharmaceutical active ingredient research.
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