发明申请
- 专利标题: SYSTEM FOR DIFFERENTIATING THE LENGTHS OF NUCLEIC ACIDS OF INTEREST IN A SAMPLE
- 专利标题(中): 用于分析样品中的核酸的长度的系统
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申请号: US12684601申请日: 2010-01-08
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公开(公告)号: US20100129896A1公开(公告)日: 2010-05-27
- 发明人: MICHAEL R. KNAPP , Jill M. Baker , Andrea W. Chow , Anne R. Kopf-Sill , Michael Spaid
- 申请人: MICHAEL R. KNAPP , Jill M. Baker , Andrea W. Chow , Anne R. Kopf-Sill , Michael Spaid
- 申请人地址: US CA Alameda
- 专利权人: CALIPER LIFE SCIENCES, INC.
- 当前专利权人: CALIPER LIFE SCIENCES, INC.
- 当前专利权人地址: US CA Alameda
- 主分类号: C12M1/00
- IPC分类号: C12M1/00
摘要:
Systems for differentiating the lengths of nucleic acids of interest in a sample are provided. The system includes a microfluidic device, a detector, and a software system. The microfluidic device includes an amplification microchannel or microchamber containing a reaction mixture under conditions that provide one or more amplicons of the nucleic acid of interest. The detector is integral with or proximal to the microfluidic device and is configured to detect the amplicons as one or more signals from a homogenous mixture. The software system interprets one or more coincidentally detected signals to indicate lengths of one or more individual nucleic acid molecules of interest, thereby differentiating the lengths of the nucleic acids of interest.