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公开(公告)号：US20140227709A1
公开(公告)日：2014-08-14
申请号：US14252755
申请日：2014-04-14
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , B01L3/5027 , B01L7/52 , B01L2200/10 , B01L2200/16 , B01L2300/1827 , B01L2400/0406 , C12Q1/6837 , C12Q1/6851 , C12Q2531/113 , C12Q2565/629 , C12Q2527/143 , C12Q2547/107 , C12Q2537/157
摘要： Methods are provided for detecting low copy nucleic acids of interest in a sample. In one method, a sample comprising a nucleic acid of interest is aliquotted into a plurality of reaction mixtures, at least two of which are single-copy reaction mixtures. The reaction mixtures are subjected to one or more amplification reactions while flowing through a channel of a microfluidic device. At least one of the reaction mixtures is formulated in an aqueous phase of an emulsion comprising aqueous droplets suspended in an immiscible liquid. The nucleic acid of interest is present as a single copy in at least one aqueous droplet of the aqueous phase prior to performing the amplification reaction(s). Amplification is performed on the reaction mixture when it is formulated in the emulsion. The nucleic acid is continuously flowed during a plurality of steps of the method.
摘要翻译： 提供了用于检测样品中感兴趣的低拷贝核酸的方法。 在一种方法中，将包含感兴趣的核酸的样品等分成多个反应混合物，其中至少两个是单拷贝反应混合物。 反应混合物在流过微流体装置的通道时进行一次或多次扩增反应。 将至少一种反应混合物配制在包含悬浮在不混溶液体中的水性液滴的乳液的水相中。 感兴趣的核酸在进行扩增反应之前以水相的至少一个水滴形式存在于单拷贝中。 当将其配制在乳液中时，对反应混合物进行扩增。 在该方法的多个步骤期间，核酸连续流动。
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2.发明授权System for differentiating the lengths of nucleic acids of interest in a sample 有权用于区分样品中感兴趣的核酸长度的系统公开(公告)号：US08275554B2
公开(公告)日：2012-09-25
申请号：US12684601
申请日：2010-01-08
IPC分类号： G01N33/48
CPC分类号： C12Q1/686 , B01L3/5027 , B01L7/52 , B01L2200/10 , B01L2200/16 , B01L2300/1827 , B01L2400/0406 , C12Q1/6837 , C12Q1/6851 , C12Q2531/113 , C12Q2565/629 , C12Q2527/143 , C12Q2547/107 , C12Q2537/157
摘要： Systems for differentiating the lengths of nucleic acids of interest in a sample are provided. The system includes a microfluidic device, a detector, and a software system. The microfluidic device includes an amplification microchannel or microchamber containing a reaction mixture under conditions that provide one or more amplicons of the nucleic acid of interest. The detector is integral with or proximal to the microfluidic device and is configured to detect the amplicons as one or more signals from a homogenous mixture. The software system interprets one or more coincidentally detected signals to indicate lengths of one or more individual nucleic acid molecules of interest, thereby differentiating the lengths of the nucleic acids of interest.
摘要翻译： 提供了用于区分样品中感兴趣的核酸长度的系统。 该系统包括微流体装置，检测器和软件系统。 微流体装置包括在提供感兴趣的核酸的一个或多个扩增子的条件下含有反应混合物的扩增微通道或微室。 检测器与微流体装置成一体或靠近微流体装置，并且被配置为将扩增子检测为来自均匀混合物的一个或多个信号。 软件系统解释一个或多个巧合检测的信号以指示感兴趣的一个或多个单个核酸分子的长度，从而区分感兴趣的核酸的长度。
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3.发明申请公开(公告)号：US20100129896A1
公开(公告)日：2010-05-27
申请号：US12684601
申请日：2010-01-08
IPC分类号： C12M1/00
CPC分类号： C12Q1/686 , B01L3/5027 , B01L7/52 , B01L2200/10 , B01L2200/16 , B01L2300/1827 , B01L2400/0406 , C12Q1/6837 , C12Q1/6851 , C12Q2531/113 , C12Q2565/629 , C12Q2527/143 , C12Q2547/107 , C12Q2537/157
摘要： Systems for differentiating the lengths of nucleic acids of interest in a sample are provided. The system includes a microfluidic device, a detector, and a software system. The microfluidic device includes an amplification microchannel or microchamber containing a reaction mixture under conditions that provide one or more amplicons of the nucleic acid of interest. The detector is integral with or proximal to the microfluidic device and is configured to detect the amplicons as one or more signals from a homogenous mixture. The software system interprets one or more coincidentally detected signals to indicate lengths of one or more individual nucleic acid molecules of interest, thereby differentiating the lengths of the nucleic acids of interest.
摘要翻译： 提供了用于区分样品中感兴趣的核酸长度的系统。 该系统包括微流体装置，检测器和软件系统。 微流体装置包括在提供感兴趣的核酸的一个或多个扩增子的条件下含有反应混合物的扩增微通道或微室。 检测器与微流体装置成一体或靠近微流体装置，并且被配置为将扩增子检测为来自均匀混合物的一个或多个信号。 软件系统解释一个或多个巧合检测的信号以指示感兴趣的一个或多个单个核酸分子的长度，从而区分感兴趣的核酸的长度。
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公开(公告)号：US07105304B1
公开(公告)日：2006-09-12
申请号：US09993314
申请日：2001-11-05
申请人： Theo T. Nikiforov , Jill M. Baker , Sansan Lin , J. Wallace Parce
发明人： Theo T. Nikiforov , Jill M. Baker , Sansan Lin , J. Wallace Parce
CPC分类号： C12Q1/485 , B01L3/5027 , C12Q1/42
摘要： Methods for chromatographically separating materials, including the separation of materials in kinase or phosphatase assays, in microfluidic devices under positive or negative fluid pressure. Devices and integrated systems for performing chromatographic separations are also provided.
摘要翻译： 用于在正或负流体压力下在微流体装置中色谱分离材料的方法，包括在激酶或磷酸酶测定中分离材料。 还提供了用于执行色谱分离的设备和集成系统。
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5.发明授权Determining nucleic acid fragmentation status by coincident detection of two labeled probes 有权通过重复检测两个标记的探针来确定核酸碎裂状态公开(公告)号：US07645581B2
公开(公告)日：2010-01-12
申请号：US11867626
申请日：2007-10-04
CPC分类号： C12Q1/6851 , B01L3/5027 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/10 , B01L2200/16 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/1822 , B01L2300/1827 , B01L2300/1833 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , C12Q1/6818 , C12Q1/6837 , C12Q1/686 , C12Q1/6869 , G01N21/6428 , G01N2021/6441 , C12Q2565/102 , C12Q2537/143 , C12Q2545/113 , C12Q2531/113 , C12Q2565/629 , C12Q2527/143
摘要： Methods for determining nucleic acid fragmentation status are provided. A nucleic acid of interest in a reaction mixture is contacted with two or more different probes complementary to sites separated by a point of potential fragmentation. The probes each comprise a detectable marker. The nucleic acid of interest is flowed into a detection region, where two or more coincident detectable marker signals from the probes are detected. Fragmentation status of the nucleic acid of interest is determined, coincident detection of signals from two or more of the different probes indicating the nucleic acid of interest is not fragmented between the probes.
摘要翻译： 提供了确定核酸碎裂状态的方法。 将反应混合物中感兴趣的核酸与两个或更多个不同的探针接触，所述探针与通过潜在断裂点分离的位点互补。 探针各自包含可检测标记。 感兴趣的核酸流入检测区域，其中检测到来自探针的两个或多个重合的可检测标记信号。 确定感兴趣的核酸的碎裂状态，来自两个或更多个指示感兴趣的核酸的不同探针的信号的一致检测在探针之间不分段。
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公开(公告)号：US08697362B2
公开(公告)日：2014-04-15
申请号：US11873753
申请日：2007-10-17
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , B01L3/5027 , B01L7/52 , B01L2200/10 , B01L2200/16 , B01L2300/1827 , B01L2400/0406 , C12Q1/6837 , C12Q1/6851 , C12Q2531/113 , C12Q2565/629 , C12Q2527/143 , C12Q2547/107 , C12Q2537/157
摘要： Methods are provided for detecting low copy nucleic acids of interest in a sample. In one method, a sample comprising a nucleic acid of interest is aliquotted into a plurality of reaction mixtures, at least two of which are single-copy reaction mixtures. The reaction mixtures are subjected to one or more amplification reactions while flowing through a channel of a microfluidic device. At least one of the reaction mixtures is formulated in an aqueous phase of an emulsion comprising aqueous droplets suspended in an immiscible liquid. The nucleic acid of interest is present as a single copy in at least one aqueous droplet of the aqueous phase prior to performing the amplification reaction(s). Amplification is performed on the reaction mixture when it is formulated in the emulsion. The nucleic acid is continuously flowed during a plurality of steps of the method.
摘要翻译： 提供了用于检测样品中感兴趣的低拷贝核酸的方法。 在一种方法中，将包含感兴趣的核酸的样品等分成多个反应混合物，其中至少两个是单拷贝反应混合物。 反应混合物在流过微流体装置的通道时进行一次或多次扩增反应。 将至少一种反应混合物配制在包含悬浮在不混溶液体中的水性液滴的乳液的水相中。 感兴趣的核酸在进行扩增反应之前以水相的至少一个水滴形式存在于单拷贝中。 当将其配制在乳液中时，对反应混合物进行扩增。 在该方法的多个步骤期间，核酸连续流动。
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