发明申请
- 专利标题: SOLUBILITY TAGS FOR THE EXPRESSION AND PURIFICATION OF BIOACTIVE PEPTIDES
- 专利标题(中): 用于表达和纯化生物活性肽的可溶性标签
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申请号: US12692665申请日: 2010-01-25
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公开(公告)号: US20100136621A1公开(公告)日: 2010-06-03
- 发明人: Qiong Cheng , Linda Jane Decarolis , Stephen R. Fahnestock , Tanja Maria Gruber , Lisa Diane Reiss , Pierre E. Rouviere
- 申请人: Qiong Cheng , Linda Jane Decarolis , Stephen R. Fahnestock , Tanja Maria Gruber , Lisa Diane Reiss , Pierre E. Rouviere
- 申请人地址: US DE Wilmington
- 专利权人: E.I. DU PONT DE NEMOURS AND COMPANY
- 当前专利权人: E.I. DU PONT DE NEMOURS AND COMPANY
- 当前专利权人地址: US DE Wilmington
- 主分类号: C12P21/00
- IPC分类号: C12P21/00 ; C07H21/00 ; C12N15/74 ; C12N1/15 ; C12N1/19 ; C12N1/21
摘要:
Peptide tags, referred to here as inclusion body tags, are disclosed useful for the generation of insoluble fusion peptides. The fusion peptides comprise at least one inclusion body tag operably linked to a peptide of interest. Expression of the fusion peptide in a host cell results in a product that is insoluble and contained within inclusion bodies in the cell and/or cell lysate. The inclusion bodies may then be purified and the protein of interest may be isolated after cleavage from the inclusion body tag.