Provided are a biochip and a biomaterial detection apparatus. The biochip includes a substrate, a metal layer, and a dielectric layer. The substrate includes a surface having a plurality of acute parts which are formed by first and second inclined planes. The metal layer is formed on at least one of the first and second inclined planes. The dielectric layer is formed on the metal layer, and capture molecules specifically binding to target molecules which are marked with a fluorescent substance are immobilized to a surface of the dielectric layer.