发明申请
US20100190224A1 ENZYME FOR THE PRODUCTION OF METHYLMALONYL-COENZYME A OR ETHYLMALONYL-COENZYME A AND USE THEREOF
审中-公开
用于生产甲基亚甲基二甲基丙烯酸酯或其乙烯基 - 苯甲醚的酶及其用途
- 专利标题: ENZYME FOR THE PRODUCTION OF METHYLMALONYL-COENZYME A OR ETHYLMALONYL-COENZYME A AND USE THEREOF
- 专利标题(中): 用于生产甲基亚甲基二甲基丙烯酸酯或其乙烯基 - 苯甲醚的酶及其用途
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申请号: US12593090申请日: 2008-03-27
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公开(公告)号: US20100190224A1公开(公告)日: 2010-07-29
- 发明人: Markus Poetter , Achim Marx , Georg Fuchs , Birgit Alber , Tobias Juergen Erb
- 申请人: Markus Poetter , Achim Marx , Georg Fuchs , Birgit Alber , Tobias Juergen Erb
- 申请人地址: DE Essen DE Freiburg
- 专利权人: EVONIK DEGUSSA Gmbh,ALBERT-LUDWIG-UNIVERSITAET FREIBURG
- 当前专利权人: EVONIK DEGUSSA Gmbh,ALBERT-LUDWIG-UNIVERSITAET FREIBURG
- 当前专利权人地址: DE Essen DE Freiburg
- 优先权: DE102007015583.4 20070329
- 国际申请: PCT/EP08/53655 WO 20080327
- 主分类号: C12P7/42
- IPC分类号: C12P7/42 ; C12N15/74 ; C12N9/00 ; C12N1/21 ; C12N15/63 ; C07H21/04
摘要:
The present invention relates to an isolated DNA, which is selected from the following sequences: a) a sequence according to SEQ ID No. 01, b) an intron-free sequence that is derived from a sequence according to a) and encodes the same protein or peptide as the sequence according to SEQ ID No. 01, c) a sequence that encodes a protein or peptide, which comprises the amino acid sequence according to SEQ ID No. 02, d) a sequence that is at least 80% identical to a sequence according to a) to c), e) a sequence that hybridizes with the antisense strand of a sequence according to one of the groups a) to d) or would hybridize taking into account degeneration of the genetic code, f) a derivative of a sequence according to one of the groups a) to e) obtained by substitution, addition, inversion and/or deletion of one or more bases, g) a sequence that corresponds to SEQ ID No. 01 within the degeneration of the genetic code, h) a sequence with neutral sense mutations of SEQ ID No. 01, and i) a sequence complementary to a sequence according to one of the groups a) to h). The invention further relates to a vector, the use of this vector for transformation of a cell, a transformed cell, a polypeptide, cells genetically engineered relative to their wild type, a method of production of a genetically engineered cell, the genetically engineered cell obtainable by this method, the use of this cell and a method of production of 3-hydroxyisobutyric acid or of a derivative thereof.
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