发明申请
US20100267571A1 NOVEL METHOD FOR SPECIMEN PREPARATION, WHICH ENSURES PRESERVATION OF TISSUE MORPHOLOGY AND NUCLEIC ACID QUALITY
审中-公开
用于样品制备的新方法，其可以保护组织形态学和核酸质量
- 专利标题： NOVEL METHOD FOR SPECIMEN PREPARATION, WHICH ENSURES PRESERVATION OF TISSUE MORPHOLOGY AND NUCLEIC ACID QUALITY
- 专利标题（中）： 用于样品制备的新方法，其可以保护组织形态学和核酸质量
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申请号： US12808142申请日： 2008-12-15
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公开(公告)号： US20100267571A1公开(公告)日： 2010-10-21
- 发明人: Takeshi Watanabe , Fang Yanting , Masami Suzuki , Hiromichi Terashima , Hideaki Mizuno
- 申请人： Takeshi Watanabe , Fang Yanting , Masami Suzuki , Hiromichi Terashima , Hideaki Mizuno
- 申请人地址： JP TOKYO SG HELIOS
- 专利权人： CHUGAI SEIYAKU KABUSHIKI KAISHA,PHARMALOGICALS RESEARCH PT. LTD.
- 当前专利权人： CHUGAI SEIYAKU KABUSHIKI KAISHA,PHARMALOGICALS RESEARCH PT. LTD.
- 当前专利权人地址： JP TOKYO SG HELIOS
- 优先权： JP2007-322944 20071214
- 国际申请： PCT/JP2008/072787 WO 20081215
- 主分类号： C40B30/00
- IPC分类号： C40B30/00 ; G01N1/36 ; G01N1/30 ; C12N5/07 ; C12Q1/68 ; C40B50/00
摘要：
The present invention aims to develop a method for specimen preparation, which ensures the maintenance of both tissue morphology and nucleic acid quality (particularly RNA quality). The present invention further aims to prepare a specimen by this method, from which desired cells are then collected by microdissection and analyzed for gene expression. A method for specimen preparation from various frozen or unfrozen organs or tissues (excluding hard tissues) of the whole body, which comprises the following steps: 1) fixing a target organ or tissue with PFA fixative; and 2) embedding the same in paraffin by the AMeX method.
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