发明申请
- 专利标题： Methods for Improving Protein Performance
- 专利标题（中）： 提高蛋白质性能的方法
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申请号： US12602985申请日： 2008-06-06
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公开(公告)号： US20100297727A1公开(公告)日： 2010-11-25
- 发明人: Wolfgang Aehle , Luis Gustavo Cascao-Pereira , James T. Kellis, JR. , Andrew Shaw
- 申请人： Wolfgang Aehle , Luis Gustavo Cascao-Pereira , James T. Kellis, JR. , Andrew Shaw
- 国际申请： PCT/US2008/007113 WO 20080606
- 主分类号： C12N9/08
- IPC分类号： C12N9/08 ; C12N9/48 ; C12N9/42 ; C12N9/26 ; C12N9/16 ; C12N9/18 ; C12N9/10 ; C12N9/02
摘要：
The present invention provides methods for engineering proteins to optimize their performance under certain environmental conditions of interest. In some embodiments, the present invention provides methods for engineering enzymes to optimize their catalytic activity under particular environmental conditions. In some preferred embodiments, the present invention provides methods for altering the net surface charge and/or surface charge distribution of enzymes {e.g., metalloproteases or serine proteases) to obtain enzyme variants that demonstrate improved performance in detergent formulations as compared to the starting or parent enzyme.
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