- 专利标题: Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site
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申请号: US11977788申请日: 2007-10-26
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公开(公告)号: US20100317096A1公开(公告)日: 2010-12-16
- 发明人: Jianmin Fang , Karin Jooss , Andrew Simmons , Jing-Jing Qian
- 申请人: Jianmin Fang , Karin Jooss , Andrew Simmons , Jing-Jing Qian
- 申请人地址: US CA South San Francisco
- 专利权人: Cell Genesys, Inc.
- 当前专利权人: Cell Genesys, Inc.
- 当前专利权人地址: US CA South San Francisco
- 主分类号: C12N15/63
- IPC分类号: C12N15/63 ; C07H21/04
摘要:
Vector constructs for expression of two or more functional proteins or polypeptides under operative control of a single promoter and methods of making and using the same are described. The vectors comprise a self-processing cleavage site between each respective protein or polypeptide coding sequence. The vector constructs include the coding sequence for a self-processing cleavage site and may further include an additional proteolytic cleavage sequence which provides a means to remove the self processing peptide sequence from expressed protein(s) or polypeptide(s). The vector constructs find utility in methods for enhanced production of biologically active proteins and polypeptides in vitro and in vivo.