发明申请
- 专利标题: ANALYSIS OF UBIQUITINATED POLYPEPTIDES
- 专利标题(中): 不饱和聚酯的分析
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申请号: US12967284申请日: 2010-12-14
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公开(公告)号: US20110111424A1公开(公告)日: 2011-05-12
- 发明人: John Edward Rush, II , Jing Li , Ailan Guo
- 申请人: John Edward Rush, II , Jing Li , Ailan Guo
- 申请人地址: US MA Danvers
- 专利权人: Cell Signaling Technology, Inc.
- 当前专利权人: Cell Signaling Technology, Inc.
- 当前专利权人地址: US MA Danvers
- 主分类号: G01N33/53
- IPC分类号: G01N33/53 ; C07K16/00
摘要:
The invention relates to antibody reagents that specifically bind to peptides carrying a ubiquitin remnant from a digested or chemically treated biological sample. The reagents allow the technician to identify ubiquitinated polypeptides as well as the sites of ubiquitination on them. The reagents are preferably employed in proteomic analysis using mass spectrometry. The antibody reagents specifically bind to the remnant of ubiquitin (i.e., a diglycine modified epsilon amine of lysine) left on a peptide which as been generated by digesting or chemically treating ubiquitinated proteins. The inventive antibody reagents' affinity to the ubiquitin remnant does not depend on the remaining amino acid sequences flanking the modified (i.e., ubiquitinated) lysine, i.e., they are context independent.