发明申请
US20110124843A1 METHOD FOR EFFICIENTLY AMPLIFYING ABNORMAL PRION PROTEIN DERIVED FROM BSE
审中-公开
有效放大从疯牛病衍生的异常蛋白质的方法
- 专利标题： METHOD FOR EFFICIENTLY AMPLIFYING ABNORMAL PRION PROTEIN DERIVED FROM BSE
- 专利标题（中）： 有效放大从疯牛病衍生的异常蛋白质的方法
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申请号： US12994589申请日： 2009-05-26
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公开(公告)号： US20110124843A1公开(公告)日： 2011-05-26
- 发明人: Yuichi Murayama , Miyako Yoshioka , Kentaro Masujin , Hiroyuki Okada , Yoshifumi Iwamaru , Morikazu Imamura , Yuichi Matsuura , Takashi Yokoyama , Shirou Mohri
- 申请人： Yuichi Murayama , Miyako Yoshioka , Kentaro Masujin , Hiroyuki Okada , Yoshifumi Iwamaru , Morikazu Imamura , Yuichi Matsuura , Takashi Yokoyama , Shirou Mohri
- 专利权人： INCORPORATED ADMINISTRATIVE AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION
- 当前专利权人： INCORPORATED ADMINISTRATIVE AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION
- 优先权： JP2008-139279 20080528
- 国际申请： PCT/JP2009/059618 WO 20090526
- 主分类号： C07K1/02
- IPC分类号： C07K1/02
摘要：
A method for efficiently amplifying abnormal prion protein (PrPSc) derived from bovine spongiform encephalopathy (BSE) is provided. Ultimately, the invention aims at eradicating the transmission of a prion disease by detecting a BSE-infected cow early and developing a method for inactivating prions and permitting early examination of prion inactivation. Provided is a method for efficiently amplifying PrPSc derived from BSE, wherein the method is based on a PMCA (protein misfolding cyclic amplification) method in which normal prion protein (PrPC) is used as a source and PrPSc is used as a seed, and PrPSc derived from BSE is amplified by stir-mixing, incubating, and sonicating both the PrPC and the PrPSc repeatedly, and wherein the method includes performing stir-mixing-incubation-sonication in the presence of a polysaccharide sulfate.
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