公开(公告)号：US20110124843A1

公开(公告)日：2011-05-26

申请号：US12994589

申请日：2009-05-26

申请人： Yuichi Murayama ,  Miyako Yoshioka ,  Kentaro Masujin ,  Hiroyuki Okada ,  Yoshifumi Iwamaru ,  Morikazu Imamura ,  Yuichi Matsuura ,  Takashi Yokoyama ,  Shirou Mohri

发明人： Yuichi Murayama ,  Miyako Yoshioka ,  Kentaro Masujin ,  Hiroyuki Okada ,  Yoshifumi Iwamaru ,  Morikazu Imamura ,  Yuichi Matsuura ,  Takashi Yokoyama ,  Shirou Mohri

IPC分类号： C07K1/02

CPC分类号： C07K14/47 ,  G01N33/6896 ,  G01N2800/2828

摘要： A method for efficiently amplifying abnormal prion protein (PrPSc) derived from bovine spongiform encephalopathy (BSE) is provided. Ultimately, the invention aims at eradicating the transmission of a prion disease by detecting a BSE-infected cow early and developing a method for inactivating prions and permitting early examination of prion inactivation. Provided is a method for efficiently amplifying PrPSc derived from BSE, wherein the method is based on a PMCA (protein misfolding cyclic amplification) method in which normal prion protein (PrPC) is used as a source and PrPSc is used as a seed, and PrPSc derived from BSE is amplified by stir-mixing, incubating, and sonicating both the PrPC and the PrPSc repeatedly, and wherein the method includes performing stir-mixing-incubation-sonication in the presence of a polysaccharide sulfate.

摘要翻译： 提供了一种有效地扩增源自牛海绵状脑病（BSE）的异常朊病毒蛋白（PrPSc）的方法。 最终本发明旨在通过早期检测BSE感染的牛并且开发一种灭活朊病毒的方法并允许早期检测朊病毒失活来消除朊病毒病的传播。 提供了一种高效地扩增BSE衍生的PrPSc的方法，其中该方法基于使用正常朊病毒蛋白（PrPC）作为来源并使用PrPSc作为种子的PMCA（蛋白质错误折叠循环扩增）法，PrPSc 通过重复搅拌，孵育和超声处理PrPC和PrPSc来扩增来自BSE的片段，并且其中所述方法包括在多糖硫酸盐存在下进行搅拌 - 温育超声处理。