Embodiments of the invention are generally directed to transmission and handling of three-dimensional video content. An embodiment of a method includes receiving a multimedia data stream including video data utilizing an interface protocol and determining that the received video data includes three-dimensional (3D) video data, where each frame of the video data includes a first vertical synchronization (Vsync) signal prior to an active data region, the active data region including a first data region and a second data region. The method further includes converting the 3D video data from a 3D data format to a two-dimensional (2D) video format, where converting the 3D video data includes identifying a region between the first data region and the second data region, inserting a second Vsync signal between the first data region and the second data region, and providing an identifier to distinguish between the first data region and the second data region.